The tetraspanins CD9 and CD81 regulate CD9P1-induced effects on cell migration.
The tetraspanins CD9 and CD81 regulate CD9P1-induced effects on cell migration.
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DOI：
10.1371/journal.pone.0011219
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发表时间：
2010-06-21
期刊：
影响因子：
3.7
通讯作者：
Le Naour F
中科院分区：
文献类型：
--
作者：
Chambrion C;Le Naour F
CD9P-1是一种具有免疫球蛋白结构域的细胞表面蛋白，其功能未知，与四跨蛋白CD9和CD81特异性相关。CD9P-1在HEK-293细胞中的过表达会引起细胞在各种基质上的扩散和迁移的剧烈变化。延时视频显微镜实验显示，CD9P-1表达通过β1-整合素依赖机制导致细胞在I型胶原上的运动性提高，而在纤维连接蛋白上的运动性降低。在I型胶原中，CD9P-1表达诱导的细胞运动增加与整合素α2β1有关，CD9P-1与该胶原受体相关。CD9P-1突变体的产生表明，跨膜和细胞质结构域是诱导细胞运动效应的必要条件。另一方面，四跨蛋白CD9或CD81的表达被证明可以逆转CD9P-1对细胞运动、胶原I或纤维连接蛋白的影响，并伴有CD9P-1的关联。因此，CD9P-1和它的tetraspanin伴侣之间的表达水平比值可以调节细胞运动。
CD9P-1 is a cell surface protein with immunoglobulin domains and an unknown function that specifically associates with tetraspanins CD9 and CD81. Overexpression of CD9P-1 in HEK-293 cells induces dramatic changes in cell spreading and migration on various matrices. Experiments using time-lapse videomicroscopy revealed that CD9P-1 expression has led to higher cell motility on collagen I but lower motility on fibronectin through a β1-integrins dependent mechanism. On collagen I, the increase in cell motility induced by CD9P-1 expression was found to involve integrin α2β1 and CD9P-1 was observed to associate with this collagen receptor. The generation of CD9P-1 mutants demonstrated that the transmembrane and the cytoplasmic domains are necessary for inducing effects on cell motility. On the other hand, expression of tetraspanins CD9 or CD81 was shown to reverse the effects of CD9P-1 on cell motility on collagen I or fibronectin with a concomitant association with CD9P-1. Thus, the ratio of expression levels between CD9P-1 and its tetraspanin partners can regulate cell motility.
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影响因子：
4.5
作者：
Garcia, E;Pion, M;Piguet, V
通讯作者：
Piguet, V
DOI：
10.1073/pnas.2536699100
发表时间：
2003-12-23
影响因子：
11.1
作者：
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影响因子：
20.3
作者：
Crew, VK;Burton, N;Anstee, DJ
通讯作者：
Anstee, DJ
影响因子：
4.3
作者：
Lagaudrière-Gesbert, C;Le Naour, F;Rubinstein, E
通讯作者：
Rubinstein, E
DOI：
10.1073/pnas.0409493102
发表时间：
2005-02-08
影响因子：
11.1
作者：
Nishiuchi, R;Sanzen, N;Sekiguchi, K
通讯作者：
Sekiguchi, K