INTEGRATION HOST FACTOR - A PROTEIN FOR ALL REASONS
INTEGRATION HOST FACTOR - A PROTEIN FOR ALL REASONS
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DOI：
10.1016/0092-8674(88)90213-9
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发表时间：
1988-11-18
期刊：
影响因子：
64.5
通讯作者：
FRIEDMAN, DI
中科院分区：
文献类型：
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作者：
FRIEDMAN, DI
大肠杆菌 DNA 结合蛋白整合宿主因子 (IHF) 的鉴定和表征是一个很好的例子，说明了如何利用已充分表征的病毒来分析宿主功能。在这种情况下，Nash 和同事通过对大肠杆菌噬菌体 h 位点特异性重组的里程碑式的体外研究（Nash，1981 年进行了回顾），发现了一种蛋白质，该蛋白质不仅在其他重组反应中发挥作用，而且在 DNA 复制和基因表达调节中发挥作用。 IHF 属于一类结构相关的“组蛋白样”蛋白质，可以将 DNA 包裹成更高级的结构（Drlica 和 Rouviere-Yaniv，1987）。大肠杆菌中最丰富的这些蛋白质是 HU，其他蛋白质已在许多细菌属和古细菌中发现。除了位点特异性重组之外，受 IHF 影响的 h 发育的其他方面也是有关该蛋白质的丰富信息来源。我首先将重点关注 h 的研究，这些研究旨在介绍有关 IHF 的基本信息，然后研究源自对大肠杆菌及其其他一些噬菌体和质粒的研究的 IHF 的各种作用。
The identification and characterization of the Escherichia coli DNA binding protein integration host factor (IHF) is an elegant example of how a well-characterized virus can be employed in the analysis of a host function. In this case, Nash and coworkers, through their landmark in vitro studies of coliphage h site-specific recombination (reiewed in Nash, 1981), have identified a protein that plays roles not only in other recombination reactions, but also in DNA replication and regulation of gene expression. IHF belongs to a class of structurally related “histonelike” proteins that can wrap DNA into higher-order structures (Drlica and Rouviere-Yaniv, 1987). The most abundant of these proteins in E. coli is HU, and others have been found in a number of bacterial genera as well as archaebacteria.In addition to site-specific recombination, other aspects of h development influenced by IHF have been fertile sources of information about this protein. I will initially focus on studies with h that serve to present the basic information about IHF and then examine the various roles for IHF derived from studies of E. coli and some of its other phages and plasmids.