Ultra-fast bright field and fluorescence imaging of the dynamics of micrometer-sized objects
Ultra-fast bright field and fluorescence imaging of the dynamics of micrometer-sized objects
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DOI：
10.1063/1.4809168
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发表时间：
2013-06-01
影响因子：
1.6
通讯作者：
Villanueva, Flordeliza S.
中科院分区：
文献类型：
--
作者：
Chen, Xucai;Wang, Jianjun;Villanueva, Flordeliza S.
高速成像在工业和科学研究的广泛领域中具有应用。在医学研究中，高速成像有可能揭示各种治疗干预措施的作用机制。例子包括超声辅助溶栓、药物递送和基因治疗。超声、微泡和生物细胞相互作用的视觉观察可能有助于理解微泡的动态行为，并可能最终导致更好地设计这种递送系统。我们提出了一个高速明场和荧光成像系统，结合外部机械波，如超声的发展。通过协同设计和合同制造，匹兹堡大学医学中心成功开发了高速成像系统。我们将该系统命名为“UPMC Cam”，指的是集成成像系统，包括多帧相机及其独特的软件控制，定制的模块化显微镜，定制的激光传输系统，其辅助超声发生器，以及用于体外和体内观察的组合超声和光学成像室。该系统能够以每秒25 × 10（6）帧的速度对显微镜明场和荧光电影成像128帧，帧大小为920 × 616像素。在超声下的微泡的示例图像示出，以证明该系统的潜在应用。(C)2013 AIP Publishing LLC.
High speed imaging has application in a wide area of industry and scientific research. In medical research, high speed imaging has the potential to reveal insight into mechanisms of action of various therapeutic interventions. Examples include ultrasound assisted thrombolysis, drug delivery, and gene therapy. Visual observation of the ultrasound, microbubble, and biological cell interaction may help the understanding of the dynamic behavior of microbubbles and may eventually lead to better design of such delivery systems. We present the development of a high speed bright field and fluorescence imaging system that incorporates external mechanical waves such as ultrasound. Through collaborative design and contract manufacturing, a high speed imaging system has been successfully developed at the University of Pittsburgh Medical Center. We named the system "UPMC Cam," to refer to the integrated imaging system that includes the multi-frame camera and its unique software control, the customized modular microscope, the customized laser delivery system, its auxiliary ultrasound generator, and the combined ultrasound and optical imaging chamber for in vitro and in vivo observations. This system is capable of imaging microscopic bright field and fluorescence movies at 25 x 10(6) frames per second for 128 frames, with a frame size of 920 x 616 pixels. Example images of microbubble under ultrasound are shown to demonstrate the potential application of the system. (C) 2013 AIP Publishing LLC.