Induction of nerve growth factor by butanol fraction of Liriope platyphylla in C6 and primary astrocyte cells
Induction of nerve growth factor by butanol fraction of Liriope platyphylla in C6 and primary astrocyte cells
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DOI：
10.1248/bpb.27.1257
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发表时间：
2004-08-01
影响因子：
2
通讯作者：
Kim, SY
中科院分区：
文献类型：
--
作者：
Hur, J;Lee, P;Kim, SY
在韩国，麦冬（LP）已被用作滋补、止咳和祛痰药多年。在这项研究中，我们发现，丁醇馏分的麦冬（BLP）条件培养基的C6和初级星形胶质细胞诱导的PC12细胞的轴突生长，并通过添加神经生长因子（NGF）抗体和GF109203X，蛋白激酶（PKC）抑制剂的效果被逆转。此外，我们证明BLP增加了NGF的表达和分泌。GF109203X还降低了C6细胞中NGF的表达。总之，我们的研究结果表明，BLP在PKC依赖的途径中增强的星形胶质细胞NGF有助于诱导PC12细胞的轴突生长。
Liriope platyphylla (LP) has been used as a tonic, antitussive, and expectorant in Korea for many years. In this study, we found that the buthanol fraction of Liriope platyphylla (BLP)-conditioned media of C6 and primary astrocyte induced the neurite outgrowth of PC12 cells, and that the effect was reversed by addition of nerve growth factor (NGF)-antibody and GF109203X, an inhibitor of protein kinase (PKC). Furthermore, we demonstrated that BLP increased the expression and secretion of NGF. GF109203X also decreased NGF expression in C6 cells. Taken together, our results suggest that astroglial NGF enhanced by BLP in a PKC-dependent pathway contributed to the induction of neurite outgrowth of PC12 cells.