A simian immunodeficiency virus macaque model of highly active antiretroviral treatment: viral latency in the periphery and the central nervous system.
A simian immunodeficiency virus macaque model of highly active antiretroviral treatment: viral latency in the periphery and the central nervous system.
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DOI：
10.1097/coh.0b013e3283412413
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发表时间：
2011-01
影响因子：
4.1
通讯作者：
Zink MC
中科院分区：
文献类型：
--
作者：
Clements JE;Gama L;Graham DR;Mankowski JL;Zink MC
在这里，研究SIV猕猴模型在确定体内HIV潜伏期和HAART期间持续病毒复制位点方面的优势。HAART治疗人群中最具特征的HIV储库是血液中的静息CD 4 + T细胞，尽管残留病毒也来自其他储库。已经开发了HAART的非人灵长类动物/SIV模型来表征潜在的HIV储库，特别是中枢神经系统（CNS）和骨髓中的干细胞，已知的和潜在的潜伏病毒储库难以在人类中研究。很少有SIV猕猴模型的HAART检查血浆和CSF病毒衰减，休息的CD 4 + T细胞窝藏复制能力潜伏SIV的数量，HAART治疗对中枢神经系统的影响，或残留的病毒复制或病毒DNA水平在该组织。使用一致的，加速SIV猕猴模型，我们的特点是外周病毒水库，包括在中枢神经系统中，HAART治疗的猕猴。SIV模型再现了全身记忆性CD 4 + T细胞的潜伏期，并表明CNS含有稳定的SIV DNA库。一个SIV猕猴模型的HAART重演病毒潜伏期，特别是在中枢神经系统中，需要研究治疗方法的功能性HIV治愈。
Here, SIV macaque models are examined for their strengths in identifying in vivo sites of HIV latency and persistent virus replication during HAART. The best-characterized HIV reservoir in HAART-treated persons is resting CD4+ T cells in blood, although residual virus also comes from other reservoirs. Nonhuman primate/SIV models of HAART have been developed to characterize potential HIV reservoirs, in particular the central nervous system (CNS) and stem cells in bone marrow, known and potential reservoirs of latent virus that are difficult to study in humans. Few SIV macaque models of HAART have examined plasma and CSF virus decay, the number of resting CD4+ T cells harboring replication-competent latent SIV, HAART-treatment effect on the CNS, or residual viral replication or viral DNA levels in that tissue. Using a consistent, accelerated SIV macaque model, we characterized peripheral viral reservoirs, including those in the CNS, among HAART-treated macaques. The SIV model reproduces latency in memory CD4+ T cells throughout the body and indicates that the CNS contains a stable SIV DNA reservoir. An SIV macaque model of HAART recapitulating viral latency, particularly in the CNS, is required to study therapeutic approaches for a functional HIV cure.