AN EXPERIMENTAL ANALYSIS OF THE DEVELOPMENT OF THE SPINAL COLUMN .3. INDUCTION OF SKELETOGENOUS CELLS
AN EXPERIMENTAL ANALYSIS OF THE DEVELOPMENT OF THE SPINAL COLUMN .3. INDUCTION OF SKELETOGENOUS CELLS
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DOI：
10.1002/jez.1401230206
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发表时间：
1953-01-01
影响因子：
--
通讯作者：
DETWILER, SR
中科院分区：
文献类型：
--
作者：
HOLTZER, H;DETWILER, SR
以下是展示会。从而得出结论，神经组织在轴性骨源性细胞的诱导中起作用，也在维持增殖中心的作用，从而为未来的背侧纵肌产生成肌细胞。移走约体节外侧部分(尾芽胚胎)的75%随后背部肌肉系统几乎完全恢复。删除等值的AMT。尽管骨骼系统是正常的，但从躯体内侧移位会导致背部肌肉的永久性缺陷。这些病例中存在的软骨来自最外侧的假定肌瘤细胞，这表明在尾芽阶段，体节的所有细胞都具有形成软骨的能力。从23-24期胚胎中摘除神经管，既抑制了中轴软骨的形成，也抑制了成肌细胞增殖中心的建立。无论有无脊索，但包括神经脊和覆盖的表皮在内的脊髓移植到体节的任何区域，都会导致一个完整的次生轴系统的诱导。该系统在组织和形态上与正常的轴突系统相同。它拥有完整的脊柱，包括关节突椎体和肋骨，双侧背侧肌块，双侧成肌细胞增殖中心，间隔系统和独特的色素沉着模式。
The following expts. lead to the conclusion that neural tissue functions in the induction of axial skeletogenous cells, and also in maintaining the proliferation center giving rise to the myoblasts for the future dorsal longitudinal muscles. Removal of approx. 75% of the lateral portion of the somite (tail-bud embryo) is followed by almost complete restitution of the dorsal musculature. Removal of an equivalent amt. from the medial side of the somite results in a permanent deficit in the dorsal muscles, though the skeletal system is normal. The cartilage present in these cases is derived from the most lateral presumptive myotomal cells, indicating that in the tail-bud stage all cells of the somite possess the potency to form cartilage. Extirpation of the neural tube from embryos in stages 23-24 inhibits both the formation of axial cartilage and the establishment of myoblast proliferation center. Grafts of spinal cord, with or without notochord, but including neural crest and overlying epidermis, into any region of the somite, results in the induction of a complete secondary axial system. This system is histologically and morphologically identical with a normal axial system. It possesses a complete vertebral column including a zygapophysial ensemble and ribs, bilateral dorsal muscle masses, bilateral myoblast proliferation centers, septal systems and characteristic pigmentation patterns.