3'UTR-dependent localization of a Purkinje cell messenger RNA in dendrites.
3'UTR-dependent localization of a Purkinje cell messenger RNA in dendrites.
复制标题
浦肯野细胞信使 RNA 在树突中的 3UTR 依赖性定位。
DOI：
10.1007/s12311-008-0051-y
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Oberdick,John
中科院分区：
文献类型：
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作者：
Zhang,Rui;Zhang,Xulun;Bian,Feng;Pu,Xin-an;Schilling,Karl;Oberdick,John
Pcp2（L7）是浦肯野细胞特异性GoLoco结构域蛋白，通过G蛋白偶联受体调节Gαi/o蛋白的活化。该途径的一个可能的下游效应是p型Ca2+通道，因此，浦肯野细胞的内在电生理可以被Pcp2调节（L7）。人们早就知道Pcp2(L7) mRNA大量定位于树突，这表明该蛋白的远端合成和局部水平变化的可能性。作为揭示这种mRNA的转运和翻译机制的第一步，我们已经开始鉴定对其在树突中的定位很重要的thecis作用序列。通过在体内表达修饰的转基因，我们发现只有65个碱基长的3'UTR在这一过程中是必需的。
Pcp2(L7) is a Purkinje cell-specific GoLoco domain protein that modulates activation of Gαi/o proteins by G protein-coupled receptors. A likely downstream effector of this pathway is the P-type Ca2+channel, and thereby, the intrinsic electrophysiology of Purkinje cells could be modulated by Pcp2(L7). It has long been known that the Pcp2(L7) mRNA is abundantly localized in dendrites, suggesting the possibility of distal synthesis and local changes in levels of the protein. As a first step to uncover the trafficking and translational mechanisms for this mRNA, we have begun identifying thecis-acting sequences important for its localization in dendrites. Using expression of modified transgenes in vivo, we show that the 3′UTR, only 65 bases long, is necessary in this process.