Protection against Dehydration: A Neutron Diffraction Study on Aqueous Solutions of a Model Peptide and Trehalose
Protection against Dehydration: A Neutron Diffraction Study on Aqueous Solutions of a Model Peptide and Trehalose
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DOI：
10.1021/acs.jpcb.8b08046
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发表时间：
2018-11-15
影响因子：
3.3
通讯作者：
Ricci, Maria Antonietta
中科院分区：
文献类型：
--
作者：
Di Gioacchino, Michael;Bruni, Fabio;Ricci, Maria Antonietta
根据干旱和正常可用水条件的变化，多种生物体可逆地经历暂停生命和活跃代谢循环的能力是一个具有挑战性的问题。随着时间的推移，人们对干旱生存自然机制的兴趣与日俱增，同时也要求更有效的生物材料保护技术。碳水化合物，例如海藻糖，积累在抗旱细胞的细胞质中，被认为是耐干燥性的原因。尽管如此，海藻糖和生物分子之间相互作用的详细描述尚未建立。中子衍射实验表明，海藻糖在模型肽 N-甲基乙酰胺的第一个壳中捕获一层水分子，但不与其直接结合。这一证据与海藻糖替代水的假设形成鲜明对比，并支持相反的观点，即海藻糖形成保护壳，在蛋白质表面捕获一层水分子，从而避免由于干旱条件造成的结构损坏。
The ability of a wide class of organisms to reversibly go through cycles of suspended life and active metabolism, depending on the turnover of drought and normal water availability conditions, represents a challenging issue. The interest in the natural mechanism for drought survival has grown over time along with the request for always more efficient conservation techniques for biological materials. Carbohydrates, such as trehalose, accumulated in the cytoplasm of drought resistant cells, are considered responsible for desiccation tolerance. Nonetheless, a detailed description of the interaction between trehalose and biomolecules is not yet established. Neutron diffraction experiments show that trehalose entraps a layer of water molecules in the first shell of a model peptide, N-methylacetamide, without direct bonding with it. This evidence contrasts the hypothesis that trehalose substitutes water and supports the opposite view, namely, of trehalose forming a protective shell which entraps a layer of water molecules at the surface of proteins, thus avoiding structural damage due to drought conditions.