A centrifugation-based method for high-throughput biomaterial separation using magnetic microbeads
A centrifugation-based method for high-throughput biomaterial separation using magnetic microbeads
复制标题
一种基于离心的磁微珠高通量生物材料分离方法
DOI：
10.1101/2023.04.26.538353
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Gotoh Yukiko
中科院分区：
文献类型：
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作者：
Sugishita Hiroki;Hojo Kazunori;Hayashi Tetsutaro;Nikaido Itoshi;Gotoh Yukiko
磁性微珠是涂有生物亲和材料的小型氧化铁纳米颗粒，可选择性地与感兴趣的特定生物分子结合，从而能够从复杂的生物样品中捕获和分离它们。磁性微珠广泛用于分子生物学各种实验中特定生物分子的纯化。然而，目前手动移液从磁性微珠中分离上清液的方法通常效率低下、耗时、劳动密集且不准确。此外，由于设备和一次性用品的成本高昂，使用专门为多孔板设计的移液机器人和液体处理器可能是一种成本高昂的方法。在这里，我们开发了一种基于离心的方法，用于从磁性微珠中高通量分离上清液。为了促进离心分离过程，我们使用了 384 Transfer Plate™（Watson，日本）和配备 384 孔磁力架的磁力架，可以轻松处理数百个样品并快速将上清液与磁性微珠分离。利用离心力驱动目标分子与磁微珠分离，样品成功分离，且回收率较高。因此，该技术提供了一种简单、快速、经济高效的生物分子分离方法，在分子生物学、临床诊断和生物技术等各个领域具有潜在的应用前景，是对现有生物分子分离方法工具箱的有价值的补充。
Magnetic microbeads are small iron oxide nanoparticles coated with a bioaffinity material that selectively binds to specific biomolecules of interest, enabling their capture and isolation from complex biological samples. Magnetic microbeads are widely used for purification of specific biomolecules in various experiments in molecular biology. However, current methods of manual pipetting to separate supernatants from magnetic microbeads are often inefficient- time-consuming, labor intensive and inaccurate. Furthermore, the use of pipetting robots and liquid handlers specifically designed for multi-well plates can be a cost-prohibitive approach due to the high cost of equipment and disposable supplies. Here, we developed a centrifugation-based method for high-throughput separation of supernatant from magnetic microbeads. To facilitate the centrifugal separation process, we used the 384 transfer plate™ (Watson, Japan) and a magnetic stand equipped with a 384-well magnetic stand, allowing easy handling of several hundred samples and rapid separation of supernatant from magnetic microbeads. The centrifugal force was used to drive the separation of target molecules from the magnetic microbeads, and sample were successfully separated with relatively high recovery rates. Thus, this technology provides a simple, rapid, and cost- and labor-effective biomolecule separation method with potential applications in various fields, including molecular biology, clinical diagnostics, and biotechnology, and is a valuable addition to the existing toolbox of biomolecule separation methods.