Plasma free amino acid profiling of five types of cancer patients and its application for early detection.
Plasma free amino acid profiling of five types of cancer patients and its application for early detection.
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DOI：
10.1371/journal.pone.0024143
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Okamoto N
中科院分区：
文献类型：
--
作者：
Miyagi Y;Higashiyama M;Gochi A;Akaike M;Ishikawa T;Miura T;Saruki N;Bando E;Kimura H;Imamura F;Moriyama M;Ikeda I;Chiba A;Oshita F;Imaizumi A;Yamamoto H;Miyano H;Horimoto K;Tochikubo O;Mitsushima T;Yamakado M;Okamoto N
最近，基于代谢组学的、易于使用的血液样本早期癌症检测方法取得了快速进展。在代谢物中，血浆游离氨基酸（PFAAs）谱分析是一种很有前途的方法，因为PFAAs连接所有器官系统并在代谢中发挥重要作用。此外，PFAA谱已知受特定疾病（包括癌症）的影响。因此，本研究的目的是确定癌症患者PFAA谱的特征，以及利用这些信息进行早期检测的可能性。血浆样本收集自来自多个研究所的大约200名患者，每位患者都被诊断患有以下五种癌症中的一种：肺癌、胃癌、结肠直肠癌、乳腺癌或前列腺癌。将患者与本研究中使用的性别和年龄匹配的对照组进行比较。采用高效液相色谱(HPLC) -电喷雾电离(ESI) -质谱（MS）法测定PFAA水平。单变量分析显示，在对照组和上述五种癌症患者之间，PFAA谱存在显著差异，即使是那些无症状的早期癌症患者。此外，多变量分析在接受者-操作者特征曲线下的面积（每种癌症的ROC AUC为>0.75）方面清楚地区分了癌症患者与对照组，而不考虑癌症分期。由于本研究设计为病例对照研究，需要进一步的调查，包括模型构建和使用更大样本量的队列验证，以确定PFAA分析的有效性。这些发现表明PFAA谱分析在改善癌症筛查和诊断以及了解疾病发病机制方面具有很大的潜力。PFAA谱也可用于从单个血液样本中确定各种疾病的诊断，这涉及相对简单的血浆测定，与现有的筛查方法相比，对受试者的身体负担较低。
Recently, rapid advances have been made in metabolomics-based, easy-to-use early cancer detection methods using blood samples. Among metabolites, profiling of plasma free amino acids (PFAAs) is a promising approach because PFAAs link all organ systems and have important roles in metabolism. Furthermore, PFAA profiles are known to be influenced by specific diseases, including cancers. Therefore, the purpose of the present study was to determine the characteristics of the PFAA profiles in cancer patients and the possibility of using this information for early detection. Plasma samples were collected from approximately 200 patients from multiple institutes, each diagnosed with one of the following five types of cancer: lung, gastric, colorectal, breast, or prostate cancer. Patients were compared to gender- and age- matched controls also used in this study. The PFAA levels were measured using high-performance liquid chromatography (HPLC)–electrospray ionization (ESI)–mass spectrometry (MS). Univariate analysis revealed significant differences in the PFAA profiles between the controls and the patients with any of the five types of cancer listed above, even those with asymptomatic early-stage disease. Furthermore, multivariate analysis clearly discriminated the cancer patients from the controls in terms of the area under the receiver-operator characteristics curve (AUC of ROC >0.75 for each cancer), regardless of cancer stage. Because this study was designed as case-control study, further investigations, including model construction and validation using cohorts with larger sample sizes, are necessary to determine the usefulness of PFAA profiling. These findings suggest that PFAA profiling has great potential for improving cancer screening and diagnosis and understanding disease pathogenesis. PFAA profiles can also be used to determine various disease diagnoses from a single blood sample, which involves a relatively simple plasma assay and imposes a lower physical burden on subjects when compared to existing screening methods.
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影响因子：
3.8
作者：
Borgan E;Sitter B;Lingjærde OC;Johnsen H;Lundgren S;Bathen TF;Sørlie T;Børresen-Dale AL;Gribbestad IS
通讯作者：
Gribbestad IS
影响因子：
3
作者：
Kim Y;Koo I;Jung BH;Chung BC;Lee D
通讯作者：
Lee D
DOI：
10.1111/j.1365-2362.1988.tb01282.x
发表时间：
1988-12-01
影响因子：
5.5
作者：
MORRISON, WL;GIBSON, JNA;RENNIE, MJ
通讯作者：
RENNIE, MJ
影响因子：
6.2
作者：
HEYMSFIELD, SB;MCMANUS, CB
通讯作者：
MCMANUS, CB
影响因子：
5.8
作者：
Loeb, Stefan;Koenigsrainer, Alfred;Terness, Peter
通讯作者：
Terness, Peter