EVOLUTION AND PROPERTIES OF AEDES-ALBOPICTUS CELL-CULTURES PERSISTENTLY INFECTED WITH SINDBIS VIRUS
EVOLUTION AND PROPERTIES OF AEDES-ALBOPICTUS CELL-CULTURES PERSISTENTLY INFECTED WITH SINDBIS VIRUS
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DOI：
10.1016/0042-6822(77)90033-2
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发表时间：
1977-01-01
期刊：
影响因子：
3.7
通讯作者：
STOLLAR, V
中科院分区：
文献类型：
--
作者：
IGARASHI, A;KOO, R;STOLLAR, V
Replicate cultures of A. albopictus cells were infected with Sindbis virus and then maintained for long periods of time by weekly subculture. During the 1st wk the viral titers ranged between 108 and 109 PFU[plaque forming units]/ml, but then gradually fell and within a few weeks stabilized at about 105 to 106 PFU/ml. Such cultures were followed for the appearance of temperature-sensitive (ts) virus, small-plaque virus and for the appearance within the cells of small (12-15 S) double-stranded viral RNA (dsRNA). Cloning experiments carried out 6 mo. or more after the initial infection showed that persistently infected cultures gave rise to virus-yielding and nonyielding clones. Similar results were obtained when virus-positive clones were recloned. Prolonged treatment of persistently infected cultures with anti-Sindbis virus serum resulted in curing of the virus infection. Cured cultures behaved in every way tested as normal uninfected A. albopictus cells. The ability to cure with anti-viral serum suggests that in this system extracellular virus is needed to perpetuate the long-term infection. After persistently infected cultures were subcultured, viral RNA synthesis and viral yields were maximal during the first 3-4 days. Thereafter, although the cell number continued to increase for several days, viral RNA synthesis and viral yields both decreased sharply. A. albopictus cells probably have efficient means for the regulation of viral biosynthesis. Although the resistance of persistently infected cultures to superinfection could be accounted for by interfering temperature-sensitive nondefective virus, the presence in these cells of 12S dsRNA suggests that defective viral genomes are also present.
