A universal primer set for PCR amplification of nuclear histone H4 genes from all animal species
A universal primer set for PCR amplification of nuclear histone H4 genes from all animal species
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DOI：
10.1093/molbev/msi053
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发表时间：
2005-03-01
影响因子：
10.7
通讯作者：
Vartanian, JP
中科院分区：
文献类型：
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作者：
Pineau, P;Henry, M;Vartanian, JP
为了控制多种动物样本的基因组DNA质量，建立了一种针对核基因的半巢式聚合酶链式反应方法。组蛋白H4基因家族由少数被认为是生物体中最保守的基因组成。研究人员对来自43个不同物种的身体和细胞的组织样本进行了研究，其中8个样本来自无脊椎动物，35个样本来自脊椎动物，涵盖所有类别。来自西伯利亚三头猛犸象(猛犸象)的古代DNA样本也进行了PCR扩增测试，这些样本发生在距今40,000到49,000年前。HIST2H4的扩增性能也与以前发表的通用PCR(28S rRNA、18S rRNA和细胞色素b)进行了比较。总体而言，95%的被研究物种产生了扩增产物，包括一些来自大猩猩和黑猩猩的旧样本。这些数据表明，HIST2H4扩增产物既适用于DNA质量检测，也适用于动物界的物种鉴定。
To control the quality of genomic DNA of samples from a wide variety of animals, a heminested PCR assay specifically targeting a nuclear gene has been developed. The histone H4 gene family comprises a small number of genes considered among the most conserved genes in living organisms. Tissue samples from necropsies and from cells belonging to 43 different species were studied, eight samples from invertebrates and 35 samples from vertebrates covering all classes. Ancient DNA samples from three Siberian woolly mammoths (Mammuthus primigenius) dating between 40,000 and 49,000 years before present were also tested for PCR amplification. Performance of HIST2H4 amplification were also compared with those of previously published universal PCRs (28S rRNA, 18S rRNA, and cytochrome b). Overall, 95% of species studied yielded an amplification product, including some old samples from gorilla and chimpanzees. The data indicate that the HIST2H4 amplimers are, thus, suitable for both DNA quality testing as well as species identification in the animal kingdom.