The comparative utility of oral swabs and probang samples for detection of foot-and-mouth disease virus infection in cattle and pigs
The comparative utility of oral swabs and probang samples for detection of foot-and-mouth disease virus infection in cattle and pigs
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DOI：
10.1016/j.vetmic.2012.09.008
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发表时间：
2013-03-23
影响因子：
3.3
通讯作者：
Belsham, Graham J.
中科院分区：
文献类型：
--
作者：
Stenfeldt, Carolina;Lohse, Louise;Belsham, Graham J.
使用定量逆转录 PCR (qRT-PCR) 检测方法，对 O 血清型 FMDV 实验感染期间从牛和猪采集的口腔拭子和 Probang 样本中测量口蹄疫病毒 (FMDV) RNA。在急性感染期间，两个物种的口腔拭子以及 Probang 样本中均可以检测到 FMDV RNA。在直接接种的动物中，从与病毒血症发生相对应的时间点开始，可以在口腔拭子和 Probang 样本中检测到 FMDV RNA，而通过接触接触感染的动物，在血清中检测到病毒 RNA 之前，口腔拭子中的 FMDV RNA 水平较低。对从持续感染 FMDV 的牛身上采集的样本进行的分析表明，尽管在感染后 10 天 (dpi) 后采集的口腔拭子中不可能检测到 FMDV RNA，尽管晚至 35 dpi 采集的 Probang 样本中也存在 FMDV RNA。牛持续感染的一个有趣特征是，感染急性期后，probang 样本中的 FMDV RNA 水平明显下降，随后 28 dpi 再次显着上升（在所有携带动物中）。这项研究的结果表明，口腔拭子的 qRT-PCR 分析是一种有效的方法，可实现牛和猪急性 FMD 的时间高效且可靠的初步诊断，而 probang 采样对于检测持续感染的牛至关重要口蹄疫病毒的“携带者”。 (C) 2012 Elsevier B.V. 保留所有权利。
Foot-and-mouth disease virus (FMDV) RNA was measured using quantitative reverse transcription-PCR (qRT-PCR) assays in oral swab and probang samples collected from cattle and pigs during experimental infections with serotype O FMDV. During acute infection, FMDV RNA was measurable in oral swabs as well as in probang samples from both species. FMDV RNA could be detected in oral swabs and probang samples from a time point corresponding to the onset of viremia in directly inoculated animals, whereas animals which were infected through contact exposure had low levels of FMDV RNA in oral swabs before viral RNA could be measured in serum. Analysis of samples collected from cattle persistently infected with FMDV showed that it was not possible to detect FMDV RNA in oral swabs harvested beyond 10 days post infection (dpi), despite the presence of FMDV RNA in probang samples that had been collected as late as 35 dpi. An interesting feature of the persistent infection in the cattle was the apparent decline in the level of FMDV RNA in probang samples after the acute phase of infection, which was followed by a marked rise again (in all the carrier animals) by 28 dpi.Results from this study indicate that qRT-PCR analysis of oral swabs is a useful approach in order to achieve a time efficient and reliable initial diagnosis of acute FMD in cattle and pigs, whereas probang sampling is essential for the detection of cattle that are persistently infected "carriers" of FMDV. (C) 2012 Elsevier B.V. All rights reserved.