Osteopontin expression in vitreous and proliferative retinal membranes of patients with proliferative vitreous retinopathy
Osteopontin expression in vitreous and proliferative retinal membranes of patients with proliferative vitreous retinopathy
复制标题
增殖性玻璃体视网膜病变患者玻璃体和增殖性视网膜膜中骨桥蛋白的表达
DOI：
10.3980/j.issn.2222-3959.2011.04.17
复制
发表时间：
2011-08-18
影响因子：
1.4
通讯作者：
Liu, Qing-Huai
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Yi;Li, Lei;Liu, Qing-Huai
目的：分析骨桥蛋白(OPN)在增生性玻璃体视网膜病变(PVR)患者玻璃体和增殖性视网膜膜中的表达。方法：收集2009年至2010年间玻璃体增生性视网膜病变(PVR)患者玻璃体积液54例，其中PVR组45例，非PVR组9例。采用双抗体夹心法测定玻璃体积液中骨桥蛋白的浓度。结果：A组玻璃体积液中骨桥蛋白含量为778.48+/-62.06 ng/m L，B组为452.99±32.52 ng/m L，A组玻璃体内骨桥蛋白含量显著高于B组，且在PVR早期随时间延长而逐渐升高。实时荧光定量RT-PCR检测增殖期视网膜组织中OPN的平均水平(F=0.14)也高于视网膜色素细胞(F=0)。结论：PVR中玻璃体和增殖性视网膜组织中OPN水平较高，提示OPN可能促进PVR的发生发展。PVR早期玻璃体OPN浓度随时间延长而升高。
AIM: To analyze osteopontin (OPN) expression in vitreous and proliferative retinal membranes of patients with proliferative vitreous retinopathy (PVR).METHODS: A total of 54 vitreous fluid samples were obtained between 2009 and 2010, which contained 45 with PVR (group A) and 9 without PVR (group B). Enzyme-linked immunosorbent assay was applied to quantify the OPN concentrations in vitreous fluid. Four samples of proliferative retinal membrane were also obtained at the time of vitrectomy, and their contents of OPN were measured by Real-time RT-PCR.RESULTS: The OPN levels in the vitreous fluid were 778.48+/- 62.06ng/mL in group A and 452.99 32.52ng/mL in group B. The vitreous OPN levels in group A were significantly higher than those in group B and to rise by time in the early stages of PVR. The average OPN levels in the proliferative retinal membranes (F =0.14) were also higher than those in the retinal pigment cells (F =0) using Real-time RT-PCR.CONCLUSION: The high vitreous and proliferative retinal membrane OPN levels in PVR suggest that OPN might promote the development of PVR. The vitreous OPN concentrations are rising by the time in the early phases of PVR.