Estrogen-like activity and dual roles in cell signaling of an Agaricus blazei Murrill mycelia-dikaryon extract
Estrogen-like activity and dual roles in cell signaling of an Agaricus blazei Murrill mycelia-dikaryon extract
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DOI：
10.1016/j.micres.2011.09.003
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发表时间：
2012-01-01
影响因子：
6.7
通讯作者：
Matsuoka, Rumiko
中科院分区：
文献类型：
--
作者：
Dong, Sijun;Furutani, Yoshiyuki;Matsuoka, Rumiko
巴西蘑菇Agaricus blazei（A. Blazei）Murrill菌丝体-双核体由于其治疗癌症的潜力而引起了全世界科学家和临床医生的注意。然而，很少有人知道它对其他疾病的影响。本研究旨在扩大A. blazei预防血管损伤并阐明其作用机制。助理Blazei提取物在基因表达谱和荧光素酶测定中均显示出雌激素样活性。事实上，提取物抑制氧化低密度脂蛋白刺激的Erk 1/2，Akt和p38在HUVEC和巨噬细胞衍生的TIB-67细胞的激活。此外，提取物增强谷胱甘肽过氧化物酶3（GPX 3）的转录。α-突触核蛋白（SNCA）和内皮型一氧化氮合酶（eNOS）基因。此外，A的摄入可减轻家兔的动脉粥样硬化病变。勃拉泽粉因此，A. Blazei可通过在细胞信号传导、抑制巨噬细胞发育和从血管损伤中恢复内皮细胞中的双重作用而用于预防动脉粥样硬化。(C)2011年爱思唯尔GrrbH。All rights reserved.
Agaricus blazei (A. blazei) Murrill mycelia-dikaryon has attracted the attention of scientists and clinicians worldwide owing to its potential for the treatment of cancer. However, little is known about its effect on other pathologies. This study sought to extend the potential medical usefulness of A. blazei for preventing vascular damage and to unravel its mechanism of action. The A. blazei extract showed estrogen-like activity in both gene expression profiling and a luciferase assay. Indeed, the extract inhibited oxidized low-density lipoprotein-stimulated activation of Erk1/2, Akt and p38 in HUVECs and macrophage-derived TIB-67 cells. Moreover, the extract enhanced transcription of the glutathione peroxidase 3 (GPX3). alpha-synuclein (SNCA) and endothelial nitrogen-oxide synthase (eNOS) genes. Furthermore, atherosclerotic lesions in rabbits were reduced by intake of A. blazei powder. Therefore, A. blazei may be useful for preventing atherosclerosis via dual roles in cell signaling, suppression of macrophage development and the recovery of endothelial cells from vascular damage. (C) 2011 Elsevier GrrbH. All rights reserved.