Octadecanoid-derived alteration of gene expression and the "Oxylipin signature" in stressed barley leaves. Implications for different signaling pathways
Octadecanoid-derived alteration of gene expression and the "Oxylipin signature" in stressed barley leaves. Implications for different signaling pathways
复制标题
DOI：
10.1104/pp.123.1.177
复制
发表时间：
2000-05-01
期刊：
影响因子：
7.4
通讯作者：
Wasternack, C
中科院分区：
文献类型：
--
作者：
Kramell, R;Miersch, O;Wasternack, C
利用气相色谱/质谱-单离子监测技术，研究了大麦叶片中应激诱导的基因表达与十八烷酸和茉莉酸盐含量的时空变化相关。山梨醇诱导的胁迫导致茉莉酸（JA）、其前体12-氧植物二烯酸（OPDA）、甲酯JAME和OPDAME含量分别短暂小幅上升，随后大幅上升。JA和JAME在12 ~ 16 h达到峰值，比OPDA和OPDAME早约4 h。然而，OPDA的累积量是其他化合物的2.5倍。二homo- ja和9,13-二脱氢- opda为次要成分。动力学分析表明，茉莉酸盐或十八烷酸盐的瞬时阈值是启动ja响应基因表达的必要和充分条件。虽然外源应用的OPDA和OPDAME代谢成JA的量很大，但它们都可以诱导基因表达，这一点可以从那些对内源形成的JA没有反应的基因中得到证明。此外，冠状碱诱导JA反应基因独立于内源JA。氘化茉莉的应用表明，茉莉的内源合成不受茉莉处理的影响。这些数据是根据不同的信号通路进行讨论的。
Stress-induced gene expression in barley (Hordeum vulgare cv Salome) leaves has been correlated with temporally changing levels of octadecanoids and jasmonates, quantified by means of gas chromatography/mass spectrometry-single ion monitoring. Application of sorbitol-induced stress led to a low and transient rise of jasmonic acid (JA), its precursor 12-oxophytodienoic acid (OPDA), and the methyl esters JAME and OPDAME, respectively, followed by a large increase in their levels. JA and JAME peaked between 12 and 16 h, about 4 h before OPDA and OPDAME. However, OPDA accumulated up to a 2.5-fold higher level than the other compounds. Dihomo-JA and 9,13-didehydro-OPDA were identified as minor components. Kinetic analyses revealed that a transient threshold of jasmonates or octadecanoids is necessary and sufficient to initiate JA-responsive gene expression. Although OPDA and OPDAME applied exogenously were metabolized to JA in considerable amounts, both of them can induce gene expression, as evidenced by those genes that did not respond to endogenously formed JA. Also, coronatine induces JA-responsive genes independently from endogenous JA. Application of deuterated JA showed that endogenous synthesis of JA is not induced by JA treatment. The data are discussed in terms of distinct signaling pathways.