Prevention of taurolithocholate-induced hepatic bile canalicular distortions by HPLC-characterized extracts of artichoke (Cynara scolymus) leaves
Prevention of taurolithocholate-induced hepatic bile canalicular distortions by HPLC-characterized extracts of artichoke (Cynara scolymus) leaves
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DOI：
10.1055/s-2002-34417
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发表时间：
2002-09-01
期刊：
影响因子：
2.7
通讯作者：
Gebhardt, R
中科院分区：
文献类型：
--
作者：
Gebhardt, R
研究了洋蓟（Cynara scolymus L.）使用电子显微镜在原代培养的大鼠肝细胞中研究了牛磺石胆酸盐诱导的胆汁淤积性胆小管膜扭曲上的叶子。当与胆汁酸同时加入时，浓度在0.08和0.5 mg/ml之间的朝鲜蓟提取物能够以剂量依赖性方式防止奇异小管膜转化的形成。然而，当肝细胞与浸提液预孵育时也发生了预防，表明不涉及胆汁酸对浸提液组分的吸收。这些结果表明，朝鲜蓟叶提取物至少在牛磺石胆酸盐的情况下发挥有效的抗胆汁淤积作用。这种作用可能有助于这种草药配方的整体保肝作用。
The effects of water-soluble extracts of artichoke (Cynara scolymus L.) leaves on taurolithocholate-induced cholestatic bile canalicular membrane distortions were studied in primary cultured rat hepatocytes using electron microscopy. Artichoke extracts at concentrations between 0.08 and 0.5 mg/ml were able to prevent the formation of bizarre canalicular membrane transformations in a dose-dependent manner when added simultaneously with the bile acid. However, prevention also occurred when the hepatocytes were preincubated with the extracts, indicating that absorption of the bile acid to components of the extracts was not involved. These results demonstrate that artichoke leaf extracts exert a potent anticholestatic action at least in the case of taurolithocholate. This effect may contribute to the overall hepatoprotective influence of this herbal formulation.