Rate Equations and Kinetic Parameters of the Reactions Involved in Pyrite Oxidation by Thiobacillus ferrooxidans
Rate Equations and Kinetic Parameters of the Reactions Involved in Pyrite Oxidation by Thiobacillus ferrooxidans
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DOI：
10.1128/aem.55.11.2918-2923.1989
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发表时间：
1989-11
影响因子：
4.4
通讯作者：
H. Lizama;Isamu Suzuki
中科院分区：
文献类型：
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作者：
H. Lizama;Isamu Suzuki
得到了黄铁矿细菌氧化过程中各反应的速率方程和动力学参数。氧化亚铁硫杆菌氧化黄铁矿的反应速率常数分别为：3.5 μM Fe ~（2+）/min/FeS_2%矿浆浓度，10 μM Fe ~（2+）/min/mM Fe ~（3+）/min，90 μM O_2/min/mg湿细胞/ml，250 μM O_2/min/mg湿细胞/ml。ferrooxidans氧化未洗涤的黄铁矿。间接浸出、T. ferrooxidans，以及未洗涤黄铁矿被T. ferrooxidans。最后一个反应被竞争性抑制细胞浓度的增加，与0.13毫克湿细胞每毫升的Ki值。T. ferrooxidans细胞也增加了Fe ~（3+）加黄铁矿的Fe ~（2+）产生速率。
Rate equations and kinetic parameters were obtained for various reactions involved in the bacterial oxidation of pyrite. The rate constants were 3.5 μM Fe2+ per min per FeS2 percent pulp density for the spontaneous pyrite dissolution, 10 μM Fe2+ per min per mM Fe3+ for the indirect leaching with Fe3+, 90 μM O2 per min per mg of wet cells per ml for the Thiobacillus ferrooxidans oxidation of washed pyrite, and 250 μM O2 per min per mg of wet cells per ml for the T. ferrooxidans oxidation of unwashed pyrite. The Km values for pyrite concentration were similar and were 1.9, 2.5, and 2.75% pulp density for indirect leaching, washed pyrite oxidation by T. ferrooxidans, and unwashed pyrite oxidation by T. ferrooxidans, respectively. The last reaction was competitively inhibited by increasing concentrations of cells, with a Ki value of 0.13 mg of wet cells per ml. T. ferrooxidans cells also increased the rate of Fe2+ production from Fe3+ plus pyrite.