Coherent anti-Stokes Raman scattering microscopy of single nanodiamonds.
Coherent anti-Stokes Raman scattering microscopy of single nanodiamonds.
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DOI：
10.1038/nnano.2014.210
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发表时间：
2014-11
影响因子：
38.3
通讯作者：
Borri P
中科院分区：
文献类型：
--
作者：
Pope I;Payne L;Zoriniants G;Thomas E;Williams O;Watson P;Langbein W;Borri P
纳米粒子作为光学标签、药物传递载体和体内造影剂在生物医学上的应用引起了极大的关注。在追求卓越的光稳定性和生物相容性的过程中，纳米金刚石（NDs）由于其独特的结构、化学、机械和光学特性而被认为是最佳选择之一。到目前为止，主要利用荧光ndds进行细胞成像。然而，它们的使用受到效率和成本的限制，无法可靠地产生具有稳定光学性质的荧光缺陷中心。在这里，我们发现单个非荧光nd在金刚石的sp3振动共振中表现出强烈的相干反斯托克斯拉曼散射（CARS）。利用相关光镜和电镜，量化了CARS信号强度与ND大小的关系。校准后的CARS信号反过来能够分析活细胞内内化的NDs的数量和大小，这为定量跟踪复杂的细胞运输途径开辟了令人兴奋的前景。
Nanoparticles have attracted enormous attention for biomedical applications as optical labels, drug delivery vehicles, and contrast agents in vivo. In the quest for superior photostability and bio-compatibility, nanodiamonds (NDs) are considered one of the best choices due to their unique structural, chemical, mechanical, and optical properties. So far, mainly fluorescent NDs have been utilized for cell imaging. However, their use is limited by the efficiency and costs in reliably producing fluorescent defect centers with stable optical properties. Here, we show that single non-fluorescing NDs exhibit strong coherent anti-Stokes Raman scattering (CARS) at the sp3 vibrational resonance of diamond. Using correlative light and electron microscopy, the relationship between CARS signal strength and ND size is quantified. The calibrated CARS signal in turn enables the analysis of the number and size of NDs internalized in living cells in situ, which opens the exciting prospect of following complex cellular trafficking pathways quantitatively.