Background norepinephrine primes astrocytic calcium responses to subsequent norepinephrine stimuli in the cerebral cortex.
Background norepinephrine primes astrocytic calcium responses to subsequent norepinephrine stimuli in the cerebral cortex.
复制标题
背景去甲肾上腺素引发星形胶质细胞钙对随后大脑皮层去甲肾上腺素刺激的反应。
DOI：
10.1016/j.bbrc.2016.12.073
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Takeuchi M and Yasui M.
中科院分区：
文献类型：
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作者：
Nuriya M;Takeuchi M and Yasui M.
大脑皮层中的去甲肾上腺素（NE）水平以两种模式调节;大脑状态与背景NE浓度的缓慢变化相关，而显著刺激诱导短暂的NE尖峰。以往的研究已经揭示了它们的不同的神经调节作用，然而，NE对星形胶质细胞活性的调节作用迄今为止还没有得到很好的表征。在这项研究中，我们评估了调制作用的背景NE对星形胶质细胞的反应，随后的刺激，使用双光子钙成像急性小鼠大脑皮层切片。我们发现，阈下背景NE显着增强随后脉冲NE刺激星形胶质细胞的钙反应。这种启动效应不依赖于神经元活动，并且由β-肾上腺素受体和下游cAMP途径的激活介导。这些结果表明，背景NE引发星形胶质细胞随后的钙响应NE刺激，并建议一种新的神经胶质细胞调节作用的大脑状态依赖性背景NE在大脑皮层。
Norepinephrine (NE) levels in the cerebral cortex are regulated in two modes; the brain state is correlated with slow changes in background NE concentration, while salient stimuli induce transient NE spikes. Previous studies have revealed their diverse neuromodulatory actions; however, the modulatory role of NE on astrocytic activity has been poorly characterized thus far. In this study, we evaluated the modulatory action of background NE on astrocytic responses to subsequent stimuli, using two-photon calcium imaging of acute murine cortical brain slices. We find that subthreshold background NE significantly augments calcium responses to subsequent pulsed NE stimulation in astrocytes. This priming effect is independent of neuronal activity and is mediated by the activation of β-adrenoceptors and the downstream cAMP pathway. These results indicate that background NE primes astrocytes for subsequent calcium responses to NE stimulation and suggest a novel gliomodulatory role for brain state-dependent background NE in the cerebral cortex.