3 GENES FOR 3 YOLK PROTEINS IN DROSOPHILA-MELANOGASTER
3 GENES FOR 3 YOLK PROTEINS IN DROSOPHILA-MELANOGASTER
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DOI：
10.1016/0014-5793(79)81138-2
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发表时间：
1979-01-01
期刊：
影响因子：
3.5
通讯作者：
BOWNES, M
中科院分区：
文献类型：
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作者：
BOWNES, M
果蝇[1]中的三种卵黄蛋白可以在其合成过程中用[35S]蛋氨酸标记，无论是在体外还是在体内[2，3]。已经表明，从果蝇脂肪体和卵巢分离的mRNA可以在无小麦生殖细胞系统中翻译成YP1和YP2，但YP3不合成[3，4]。因此，不知道小麦胚芽是否只是不能翻译YP3的mRNA，或者YP3是否来源于YP1、YP2或更大的前体。我们没有发现前体的证据，此外，肽分析和在血淋巴和卵巢中YPl量减少的突变体中YP3的存在[5]表明YP3是YP2的修饰或从单独的mRNA翻译。在这篇论文中，短的标记周期被用于“体内”和“体外”，以表明YP3不太可能是另一种蛋白质的衍生物。
The three yolk proteins in Drosophilamelanogaster [1] can be labelled with [35S] methionine during their synthesis either ‘in vitro’or ‘in vivo’[2, 3]. It has been shown that mRNA isolated from the fat body and ovaries of DrosophiZa can be translated in the wheat germ cell free system into YPl and YP2, yet YP3 was not synthesised [3, 4]. Consequently it was not known whether the wheat germ simply failed to translate the mRNA for YP3 or if perhaps YP3 was derived from YPl, YP2, or a larger precursor. We found no evidence of a precursor, and furthermore peptide analysis and the presence of YP3 in a mutant with reduced quantities of YPl in the haemolymph and ovary [5] indicated that YP3 is either a modification of YP2 or is translated from a separate mRNA. In this paper short labelling periods are used both ‘in vivo’and ‘in vitro’to indicate that is in unlikely that YP3 is a derivative of another protein.