The nanocomposite scaffold of poly(lactide-co-glycolide) and hydroxyapatite surface-grafted with L-lactic acid oligomer for bone repair
The nanocomposite scaffold of poly(lactide-co-glycolide) and hydroxyapatite surface-grafted with L-lactic acid oligomer for bone repair
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DOI：
10.1016/j.actbio.2009.03.024
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发表时间：
2009-09-01
影响因子：
9.7
通讯作者：
Chen, Xuesi
中科院分区：
文献类型：
--
作者：
Cui, Yang;Liu, Yi;Chen, Xuesi
将LAc低聚物接枝到羟基磷灰石（HA）表面，制备了L-乳酸低聚物（LAc低聚物）表面修饰的纳米羟基磷灰石（op-HA）。采用熔融成型和颗粒浸出的方法将复合材料中op-HA含量分别为5、10、20和40 wt.%的op-HA/PLGA纳米复合材料制成三维支架。 PLGA 和 HA/PLGA 纳米复合材料与 10 wt.% 未接枝羟基磷灰石用作对照。支架具有高度多孔性，孔结构分布均匀且相互连通，孔隙率在90%左右。 op-HA/PLGA 复合材料中，除了 NaCl 颗粒浸出产生的 100-300 μm 大孔外，孔壁中的微孔（1-50 μm）随着 op-HA 含量的增加而增加。 op-HA 颗粒在 PLGA 基质中比纯 HA 颗粒分散得更均匀。 20 wt.% op-HA/PLGA 样品表现出最大的机械强度，包括弯曲强度 (4.14 MPa) 和压缩强度 (2.31 MPa)。 10 wt.% op-HA/PLGA 和 20 wt.% op-HA/PLGA 薄膜上的细胞活力和附着成骨细胞面积明显高于其他复合材料。在动物试验中，用 10 和 20 wt.% op-HA/PLGA 治疗的缺损快速愈合，术后 24 周观察到大骨痂桥接。植入PLGA 的组和未治疗组均存在桡骨缺损不愈合的情况。这通过组织学分析的马森三色染色显微照片得到证实。所有数据推断，op-HA 含量为 10 和 20 wt.% 的复合材料表现出更好的综合性能，是骨修复的最佳复合材料。 (C) 2009 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Nanohydroxyapatite (op-HA) surface-modified with L-lactic acid oligomer (LAc oligomer) was prepared by LAc oligomer grafted onto the hydroxyapatite (HA) surface. The nanocomposite of op-HA/PLGA with different op-HA contents of 5, 10, 20 and 40 wt.% in the composite was fabricated into three-dimensional scaffolds by the melt-molding and particulate leaching methods. PLGA and the nanocomposite of HA/PLGA with 10 wt.% of ungrafted hydroxyapatite were used as the controls. The scaffolds were highly porous with evenly distributed and interconnected pore structures, and the porosity was around 90%. Besides the macropores of 100-300 mu m created by the leaching of NaCl particles, the micropores (1-50 mu m) in the pore walls increased with increasing content of op-HA in the composites of op-HA/PLGA. The op-HA particles could disperse more uniformly than those of pure HA in PLGA matrix. The 20 wt.% op-HA/PLGA sample exhibited the maximum mechanical strength, including bending strength (4.14 MPa) and compressive strength (2.31 MPa). The cell viability and the areas of the attached osteoblasts on the films of 10 wt.% op-HA/PLGA and 20 wt.% op-HA/PLGA were evidently higher than those on the other composites. For the animal test, there was rapid healing in the defects treated with 10 and 20 wt.% op-HA/PLGA, where bridging by a large bony callus was observed at 24 weeks post-surgery. There was nonunion of radius defects implanted with PLGA and in the untreated group. This was verified by the Masson's trichrome staining photomicrographs of histological analysis. All the data extrapolated that the composite with 10 and 20 wt.% op-HA exhibited better comprehensive properties and were the optimal composites for bone repairing. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.