Long-distance transportation of live isolated lymphatic vessels.
Long-distance transportation of live isolated lymphatic vessels.
复制标题
活体分离淋巴管的长途运输。
DOI：
10.1089/lrb.2010.0007
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发表时间：
2010
影响因子：
1.4
通讯作者：
Davis,MichaelJ
中科院分区：
文献类型：
--
作者：
Gashev,AnatoliyA;Davis,MichaelJ
背景：我们进行了实验，以测试分离的淋巴管在隔夜长途运输后是否仍能存活。方法与结果：将新鲜分离的大鼠肠系膜淋巴管置入经冷冻D-MEM/F12填充并添加抗生素的管中，通过快递连夜从德克萨斯州的College Station运送到密苏里州的Columbia，进行标准的生理和药理学试验，研究长途运输过程对分离、插管和加压的大鼠肠系膜淋巴管自发收缩性的影响。结果表明，如果采取适当的预防措施，在长距离运输和随后的夜间恢复中，可以保持离体淋巴管的正常收缩功能。结论：本报告中描述的淋巴管运输方法为在偏远地区采集和最初处理的淋巴组织进行体外功能测试提供了机会。此外，所描述的程序可能会扩大在手术或尸检中收获的新鲜人体组织的潜在来源的选择，并允许在偏远地区的淋巴研究人员使用。
Background:We performed experiments to test whether isolated lymphatic vessels would remain viable after overnight long-distance shipment.Methods and Results:Freshly isolated segments of rat mesenteric lymphatic vessels were placed into tubes filled by chilled D-MEM/F12 supplemented with antibiotics and shipped via overnight by express mail from College Station, TX to Columbia, MO. Standard physiological and pharmacological tests were performed to investigate the influence of the long-distance transport procedure on the spontaneous contractility of isolated, cannulated, and pressurized rat mesenteric lymphatic vessels. The results demonstrate that normal contractile function of isolated lymphatic vessels can be preserved with long-distance shipping and subsequent overnight recovery if the proper precautions are taken.Conclusions:The method of lymphatic vessel transportation described in this report opens up the opportunity to performin vitrofunctional tests on lymphatic tissues harvested and initially processed in a remote location. In addition, the described procedures may expand the options for potential sources of fresh human tissue, harvested during surgery or autopsy and allowed to be available to lymphatic researchers in remote locations.