Construction and identification of a bacterial plasmid containing the human fibroblast interferon gene sequence.
Construction and identification of a bacterial plasmid containing the human fibroblast interferon gene sequence.
复制标题
含有人成纤维细胞干扰素基因序列的细菌质粒的构建和鉴定。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
T. Sudo
中科院分区：
文献类型：
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作者：
T. Taniguchi;M. Sakai;Y. Fujii‐Kuriyama;M. Muramatsu;Shigeyasu Kobayashi;T. Sudo
导论.干扰素是物种特异性糖蛋白（Weil和Dorner，1973），其由各种细胞在病毒和双链RNA以及其他化合物诱导后产生，在受体细胞中赋予抗病毒状态（Isaacs和Lindenmann，1957）。除了它们的抗病毒功能之外，还描述了其他多种功能，例如抗肿瘤和抗细胞功能（参见The Interferon System，1977的综述）。人血沉棕黄层细胞培养物和人成纤维细胞培养物中产生的干扰素表现出许多不同的性质（Havell等人，1975;贝格等人，1975; Gresser等人，1974 ; Vilcek等人，1977），表明白细胞干扰素和成纤维细胞干扰素是由不同的结构基因编码的不同蛋白质（Cavalieri等，1977年）。已经进行了许多尝试，用于干扰素分子的物理和化学表征及其临床研究。然而，主要是由于细胞产生的这种蛋白质的量低，因此难以获得足够量的纯化干扰素用于这些研究。解决这个问题的一种方法似乎是使用重组DNA技术。正如许多蛋白质的情况一样，克隆编码干扰素的基因将为这种蛋白质的分子结构以及基因的组织提供明确的答案。此外，在基因操作的帮助下，克隆的DNA可以在细菌或同源宿主中作为有效的干扰素生产者。在这里，我们提出的证据，成功地构建了一个混合质粒DNA含有人成纤维细胞干扰素基因序列。材料和方法。我们分离到一株干扰素高产菌DIP 2。超诱导
Introduction. Interferons are species specific glycoproteins (Weil and Dorner, 1973) which are produced by various cells upon induction with viruses and double-stranded RNAs as well as other compounds, imparting an antiviral state in the recipient cells (Isaacs and Lindenmann, 1957). Beside their antiviral function, other multiple functions such as antitumor and anticellular functions have been described (see The Interferon System, 1977 for reviews). Interferons produced in cultures of human buffy coat cells and in human fibroblasts exhibit a number of distinct properties (Havell et at., 1975; Berg et at., 1975; Gresser et at., 1974 ; Vilcek et at.,1977) , suggesting that leucocyte interferon and fibroblast interferon are different proteins coded for by distinct structural genes (Cavalieri et at., 1977). A number of attempts have been made for physical and chemical characterization of the interferon molecule and for its clinical investigation. However, mainly due to the low amount of this protein produced by the cell, it is as yet difficult to obtain sufficient quantities of purified interferon for these studies. One approach to solve the problem appears to be the use of recombinant DNA technology. As has been the case for many proteins, cloning of the gene coding for interferon will provide a definitive answer to the molecular structure of this protein as well as the organization of the gene. In addition, with the aid of gene manipulation, cloned DNA may serve as a potent interferon producer in bacterial or in cognate host. Here, we present evidence for successful construction of a hybrid plasmid DNA containing a human fibroblast interferon gene sequence. Materials and methods. Human foreskin fibroblast strain DIP2, a high interferon producer, has been isolated by us. Superinduction