Measurement of endogenous carbon monoxide formation in biological systems.
Measurement of endogenous carbon monoxide formation in biological systems.
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DOI：
10.1089/152308602753666325
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发表时间：
2002-04
影响因子：
6.6
通讯作者：
G. S. Marks;H. Vreman;B. McLaughlin;J. Brien;K. Nakatsu
中科院分区：
文献类型：
--
作者：
G. S. Marks;H. Vreman;B. McLaughlin;J. Brien;K. Nakatsu
内源性一氧化碳（CO）的形成已经测量在不同的生物系统使用各种分析程序。方法包括气相色谱-还原气相检测，气相色谱-质谱检测，激光传感器-红外吸收，紫外可见分光光度法测量CO-血红蛋白或CO-肌红蛋白复合物，以及[2-(14)C]甘氨酸后形成的（14）C-血红素生成（14）CO。从足月人胎盘绒毛膜绒毛的低0.029 nmol/mg蛋白/h到培养的大鼠嗅觉受体神经元和大鼠肝脏灌注液的高0.28 nmol/mg蛋白/h的CO形成范围不等。
Endogenous carbon monoxide (CO) formation has been measured in different biological systems using a variety of analytical procedures. The methods include gas chromatography-reduction gas detection, gas chromatography-mass spectroscopic detection, laser sensor-infrared absorption, UV-visible spectrophotometric measurement of CO-hemoglobin or CO-myoglobin complex, and formation of (14)CO from (14)C-heme formed following [2-(14)C]glycine administration. CO formation ranged from a low of 0.029 nmol/mg of protein/h in chorionic villi of term human placenta to a high of 0.28 nmol/mg of protein/h in rat olfactory receptor neurons in culture and rat liver perfusate.