Three distinct forms of atrial natriuretic factor receptors: kidney tubular epithelium cells and vascular smooth muscle cells contain different types of receptors.
Three distinct forms of atrial natriuretic factor receptors: kidney tubular epithelium cells and vascular smooth muscle cells contain different types of receptors.
复制标题
心房钠尿因子受体的三种不同形式：肾小管上皮细胞和血管平滑肌细胞含有不同类型的受体。
DOI：
10.1016/s0006-291x(87)80189-4
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Misono,KS
中科院分区：
文献类型：
--
作者：
Pandey,KN;Inagami,T;Misono,KS
从培养的犬肾小管（MDCK）细胞和大鼠胸主动脉平滑肌（RTASM）细胞中鉴定出三种不同的心钠素受体亚型。这三种ANF受体亚型包括：（1）在RTASM细胞中发现的二硫键连接的140 kDa蛋白质，其被巯基试剂二硫苏糖醇（DTT）还原为70 kDa条带，（2）二硫键未连接的120 kDa蛋白质，特异于其Mr不被DTT降低的MDCK细胞和（3）68-RTASM和MDCK细胞中普遍存在的70 kDa蛋白，其Mr未被DTT降低。不可还原的68-70 kDa和可还原的140 kDa蛋白质对全长ANF（99 -126）和截短的ANF（103-123）肽显示出强亲和力，然而，不可还原的120 kDa蛋白质仅对全长ANF（99-126）显示出强亲和力，但对截短的ANF（103-123）显示出可忽略的或非常弱的亲和力。这些结果表明，不同的心钠素受体亚型存在于肾脏和血管细胞，这可能与心钠素的多种生理功能，如尿钠排泄和利尿在肾脏和血管平滑肌细胞的血管舒张。
Three distinct ANF receptor subtypes have been identified and characterized from cultured canine kidney tubular (MDCK) cells and rat thoracic aortic smooth muscle (RTASM) cells. These three ANF receptor subtypes include; (1) a disulfide-linked 140 kDa protein found in RTASM cells which was reduced by sulfhydryl reagent dithiothreitol (DTT) to a 70 kDa band, (2) a disulfide-unlinked 120 kDa protein, specific to MDCK cells whose Mr was not reduced by DTT and (3) a 68–70 kDa protein prevalent in both RTASM and MDCK cells whose Mr was not reduced by DTT. The non-reducible 68–70 kDa and the reducible 140 kDa proteins showed strong affinities to the full-length ANF99–126)and truncated ANF(103–123)peptides, however, non-reducible 120 kDa protein showed strong affinity only to the full length ANF(99–126)but negligible or very weak affinity to truncated ANF(103–123). These findings suggest that distinct ANF receptor subtypes are present in renal and vascular cells which might be linked to diverse physiological functions of ANF such as natriuresis and diuresis in kidney and vasorelaxation in vascular smooth muscle cells.