Online oxygen measurements in ex vivo perfused muscle tissue in a porcine model using dynamic quenching methods
Online oxygen measurements in ex vivo perfused muscle tissue in a porcine model using dynamic quenching methods
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DOI：
10.1007/s00402-011-1458-3
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发表时间：
2012-05-01
影响因子：
2.3
通讯作者：
Praebst, Konstantin
中科院分区：
文献类型：
--
作者：
Dragu, Adrian;Taeger, Christian D.;Praebst, Konstantin
自体游离组织瓣移植是治疗大而复杂的组织缺损的最佳适用技术，但仍然有一个主要的失败标准。组织，特别是肌肉组织，对缺血非常敏感，因此在缺氧的关键时期后，皮瓣部分或全部丢失的风险很高。因此，开发了一种小型化的离体灌注系统，该系统可以在操作延迟期间为组织提供供应。本研究的目的是确定使用不同灌注液的灌注过程中的氧合水平，从而客观化是否需要补充氧合装置来提高灌注质量。使用基于动态猝灭的微创光学氧传感器测量组织以及灌注液的氧含量。离体灌注组织为猪腹直肌。结果表明，在用肝素化晶体液（Jonosteril(A(R))）和肝素化自体全血灌注期间，灌注反应器的额外氧合导致不同的离体氧组织饱和度，这可以通过动态猝灭来检测。动态猝灭方法是在猪模型中对离体灌注肌肉组织进行在线氧测量的一种有前途且有价值的技术。
Transplantation of autologous free tissue flaps is the best applicable technique for treating large and complex tissue defects and still has one major failure criterion. Tissue-and in particular muscle tissue-is strongly sensitive to ischemia, thus after a critical period of oxygen depletion the risk of a partial or total flap loss is high.For that reason a miniaturized ex vivo perfusion system has been developed, that supplies the tissue during operational delays. The purpose of this study was to determine the oxygenation levels during such a perfusion using different perfusates and therefore to objectify if a complementary oxygenation unit is required to improve perfusion quality. The oxygen levels of the tissue, as well of the perfusate, were measured by using minimal invasive optical oxygen sensors that are based on dynamic quenching. The ex vivo perfused tissue was the porcine rectus abdominis muscle.Results show, that during perfusion with heparinized crystalloid fluid (Jonosteril(A (R))) and heparinized autologous whole blood, additional oxygenation of the perfusion reactor led to different ex vivo oxygen tissue saturations, which can be detected by dynamic quenching.Dynamic quenching methods are a promising and valuable technique to perform online oxygen measurements in ex vivo perfused muscle tissue in a porcine model.