High-Performance Liquid Chromatographic Determination of Fluvoxamine and Fluvoxamino Acid in Human Plasma
High-Performance Liquid Chromatographic Determination of Fluvoxamine and Fluvoxamino Acid in Human Plasma
复制标题
高效液相色谱法测定人血浆中的氟伏沙明和氟伏沙氨基酸
作者：
T. Ohkubo;R. Shimoyama;K. Otani;Keizo Yoshida;H. Higuchi;T. Shimizu
建立了同时测定血浆中氟伏沙明及其主要代谢物氟伏沙氨基酸浓度的高效液相色谱法。血浆中氟伏沙明和氟伏沙氨基酸用C_18键合固相萃取柱提取，C_4反相高效液相色谱分离。以氟伏沙明、氟伏沙氨基酸和莫哌隆为内标物，在254 nm处进行紫外吸光度测定。可以分别在25.0-200.0 ng/mL浓度范围内测定氟伏沙明和氟伏沙氨基酸。氟伏沙明和氟伏沙氨基酸的检出限分别为10.0 ng/mL。血浆中氟伏沙明和氟伏沙氨基酸的平均回收率分别大于94.0%和96.5%，变异系数分别小于7.6%和8.2%。该方法已用于同时测定接受200 mg口服氟伏沙明（每日两次，剂量为100 mg）治疗的抑郁症患者中氟伏沙明和氟伏沙氨基酸的稳态血浆浓度（Css）。12例日本患者的氟伏沙明和氟伏沙明氨基酸的Css值存在个体差异，其范围分别为48.3-532.9 ng/ml和35.6-307.1 ng/ml。
A high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of fluvoxamine and its major metabolite fluvoxamino acid in plasma. Fluvoxamine and fluvoxamino acid in plasma were extracted using a C_18 bonded-solid phase cartridge, followed by C_4 reversed-phase HPLC separation. Fluvoxamine, fluvoxamino acid and moperone as an internal standard were detected by ultraviolet absorbance at 254 nm. It was possible to determine both fluvoxamine and fluvoxamino acid in the concentration range of 25.0–200.0 ng/mL, respectively. The detection limits of both fluvoxamine and fluvoxamino acid were 10.0 ng/mL, respectively. The mean recoveries of fluvoxamine and fluvoxamino acid added to plasma were more than 94.0% and 96.5%, with a coefficient of variation of less than 7.6% and 8.2%, respectively. This method has been used for the simultaneous determination of steady-state plasma concentration (Css) of fluvoxamine and fluvoxamino acid in depressive patients treated with 200 mg of oral fluvoxamine dosed as 100 mg twice-daily. The Css values of fluvoxamine and fluvoxamino acid in twelve Japanese patients were showed individual variations, which were in the range of 48.3–532.9 ng/ml and 35.6–307.1 ng/ml, respectively.
反相液相色谱法测定血浆中氟伏沙明的浓度。
DOI：
10.1002/bmc.1130080605
发表时间：
1994
期刊：
Biomedical chromatography : BMC
影响因子：
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作者：
Wong,SH;Kranzler,HR;DellaFera,S;Fernandes,R
通讯作者：
Fernandes,R