[18F]FDG uptake and PCNA, glut-1, and hexokinase-II expressions in ccancers and inflammatory lesions of the lung
[18F]FDG uptake and PCNA, glut-1, and hexokinase-II expressions in ccancers and inflammatory lesions of the lung
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DOI：
10.1593/neo.04577
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发表时间：
2005-04-01
期刊：
影响因子：
4.8
通讯作者：
Saga, T
中科院分区：
文献类型：
--
作者：
Mamede, M;Higashi, T;Saga, T
目得：本研究的目的是评估[F-18]氟脱氧葡萄糖（[F-18]-FDG）摄取、Glut-1和HK-II表达与切除肺病变炎症分级之间的关系。材料和方法：60例患者接受术前F-18-FDG-PET成像和开胸手术。对于F-18-FDG吸收的半定量分析，计算了部分体积效应校正的最大标准化吸收值（pSUV）。使用抗Glut-1、抗HK-II和抗增殖细胞核抗原（PCNA）抗体对切除标本进行免疫组织化学染色，并将免疫反应性以五分制评分为G-、H-和P-指数（0：0%; 1：类似于20%，2：类似于40%; 3：类似于60%; 4：类似于80%，5：类似于100%强免疫反应性细胞百分比）。还评价了炎症等级。结果：恶性病变的pSUV值高于非恶性病变，G指数和H指数也高于非恶性病变。pSUV与G-（p
PURPOSE: The aim of this study was to evaluate the relationships among [F-18]fluorodeoxyglucose ([F-18]-FDG) uptake, Glut-1 and HK-II expressions, and grade of inflammation in resected lung lesions. MATERIALS AND METHODS: Sixty patients had undergone preoperative F-18-FDG-PET imaging and thoracotomy. For semi-quantitative analysis of F-18-FDG uptake, partial volume effect corrected maximum standardized uptake values (pSUVs) were calculated. Immunohistochemical staining was performed in resected specimens using anti-Glut-1, anti-HK-II, and anti-proliferative cellular nuclear antigen (PCNA) antibodies, and immunoreactivities were scored as G-, H-, and P-indexes on a five-point scale (0: 0%; 1: similar to 20%, 2: similar to 40%; 3: similar to 60%; 4: similar to 80%, and 5: similar to 100% percentages of strongly immunoreactive cells). Grade of inflammation was also evaluated. RESULTS: The malignant lesions had higher pSUV and higher G- and H-indexes than nonmalignant lesions. pSUVs correlated with the G- (p