Isolation and analysis of candidate myeloid tumor suppressor genes from a commonly deleted segment of 7q22
Isolation and analysis of candidate myeloid tumor suppressor genes from a commonly deleted segment of 7q22
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DOI：
10.1016/j.ygeno.2005.01.013
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发表时间：
2005-05-01
期刊：
影响因子：
4.4
通讯作者：
Shannon, KM
中科院分区：
文献类型：
--
作者：
Curtiss, NP;Bonifas, JM;Shannon, KM
7号单体和7q缺失是复发性白血病相关的细胞遗传学异常，与儿童和成人的不良后果相关。我们描述了一个2.52 mb的基因组DNA序列，它跨越了在髓系恶性肿瘤中发现的染色体带7q22的一个常见缺失片段。该区间目前包括14个基因，19个预测基因，5个预测假基因。我们广泛表征了FBXL13、NAPE-PLD和SVH基因作为候选髓系肿瘤抑制因子。FBXL13编码一种新的F-box蛋白，SVHis是含有犰狳样重复序列的基因家族成员，NAPE-PLD编码一种磷脂酶d型磷酸二酯酶。对一组单体7白血病标本的分析未发现这些或候选基因LRRC17、PRO1598和SRPK2发生突变。这个完全测序和注释的contig为候选髓系肿瘤抑制基因的发现提供了资源。(c) 2005爱思唯尔公司版权所有。
Monosomy 7 and deletions of 7q are recurring leukemia-associated cytogenetic abnormalities that correlate with adverse outcomes in children and adults. We describe a 2.52-Mb genomic DNA contig that spans a commonly deleted segment of chromosome band 7q22 identified in myeloid malignancies. This interval currently includes 14 genes, 19 predicted genes, and 5 predicted pseudogenes. We have extensively characterized the FBXL13, NAPE-PLD, and SVH genes as candidate myeloid tumor suppressors. FBXL13 encodes a novel F-box protein, SVHis a member of a gene family that contains Armadillo-like repeats, and NAPE-PLD encodes a phospholipase D-type phosphodiesterase. Analysis of a panel of leukemia specimens with monosomy 7 did not reveal mutations in these or in the candidate genes LRRC17, PRO1598,and SRPK2. This fully sequenced and annotated contig provides a resource for candidate myeloid tumor suppressor gene discovery. (c) 2005 Elsevier Inc. All rights reserved.