Three-dimensional (3D) reconstruction and quantitative analysis of the microvasculature in medulloblastoma and ependymoma subtypes
Three-dimensional (3D) reconstruction and quantitative analysis of the microvasculature in medulloblastoma and ependymoma subtypes
复制标题
DOI：
10.1007/s10456-006-9054-9
复制
发表时间：
2006-01-01
期刊：
影响因子：
9.8
通讯作者：
Wesseling, Pieter
中科院分区：
文献类型：
--
作者：
Gilhuis, H. Jacobus;van der Laak, Jeroen A. W. M.;Wesseling, Pieter
在世界卫生组织（WHO）的神经系统肿瘤分类中，确认了髓母细胞瘤的四种主要组织病理学亚型（经典型髓母细胞瘤、促结缔组织增生性髓母细胞瘤、具有广泛结节性和晚期神经元分化的髓母细胞瘤和大细胞/间变性髓母细胞瘤）以及室管膜肿瘤（低度室管膜瘤、间变性室管膜瘤、粘液乳头状室管膜瘤和室管膜下瘤）。假设肿瘤的微血管结构是组织病理学亚型的反映，我们进行了三维重建的微血管在这些亚型的髓母细胞瘤和室管膜肿瘤使用计算机图像分析。此外，我们定量评估了三个微血管参数（数量，面积，周长）在这些肿瘤。三维重建显示在经典型和大细胞型髓母细胞瘤中有密集的不规则血管。在促结缔组织增生性髓母细胞瘤和广泛结节化的髓母细胞瘤中，血管在结节区域周围分布和组织更不均匀。典型髓母细胞瘤和大细胞髓母细胞瘤的平均血管面积和周长最大。典型髓母细胞瘤和广泛结节化的髓母细胞瘤血管数最多。室管膜肿瘤的三维分析显示，低级别室管膜瘤与间变性室管膜瘤相比，血管更大但更少，而粘液乳头状室管膜瘤血管复杂，不均匀，室管膜下瘤血管少但规则。在室管膜肿瘤中，血管数目、血管面积和血管周长的最高值见于间变性室管膜瘤，而最低值见于室管膜下瘤。我们的结论是，我们的三维重建揭示了前所未有的肿瘤血管的髓母细胞瘤和室管膜肿瘤。并期望这种重建是进一步研究肿瘤血管生成的有用工具。
In the World Health Organisation (WHO) classification of tumours of the nervous system, four main histopathological subtypes of medulloblastomas (classic medulloblastoma, desmoplastic medulloblastoma, medulloblastoma with extensive nodularity and advanced neuronal differentiation and large cell/anaplastic medulloblastoma) as well as of ependymal tumours (low-grade ependymoma, anaplastic ependymoma, myxopapillary ependymoma and sube-pendymoma) are recognised. Under the hypothesis that the microvascular architecture of tumours is a reflection of the histopathological subtype, we performed three-dimensional reconstructions of the microvasculature in these subtypes of medulloblastomas and ependymal tumours using computerised image analysis. In addition, we quantitatively assessed three microvascular parameters (number, area, perimeter) in these neoplasms. Three-dimensional reconstructions showed a dense pattern of irregular vessels in classic and large cell medulloblastoma. In desmoplastic medulloblastoma and medulloblastoma with extensive nodularity, the vessels were more unevenly distributed and organised around the nodular areas. Classic medulloblastoma and large cell medulloblastoma had on average the largest vessel area and perimeter. The highest number of vessels was seen in classic medulloblastoma and medulloblastoma with extensive nodularity. Three-dimensional analysis of ependymal tumours showed that low-grade ependymoma had larger but fewer vessels compared to anaplastic ependymoma, while myxopapillary ependymoma had a complex, heterogeneous pattern of vessels and subependymoma few but regular vessels. In ependymal tumours, the highest values for vessel number, vessel area and vessel perimeter were found in anaplastic ependymoma and the lowest values in subependymoma. We conclude that our three-dimensional reconstructions shed unprecedented light on the tumour vasculature in medulloblastomas and ependymal tumours. and expect that such reconstructions are helpful tools for further studies on tumour angiogenesis.