An inhibitor of sodium transport in the urine of dogs with normal renal function.
An inhibitor of sodium transport in the urine of dogs with normal renal function.
复制标题
肾功能正常的狗尿液中钠转运的抑制剂。
DOI：
10.1172/jci108206
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
J. Bourgoignie
中科院分区：
文献类型：
--
作者：
H. Favre;K. H. Hwang;R. Schmidt;N. Bricker;J. Bourgoignie
慢性尿毒症患者和狗的尿液和血清中含有钠转运抑制剂，可降低蟾蜍膀胱中的短路电流（SCC），并在大鼠中产生尿钠排泄。目前的研究代表了一种努力，以确定是否相同的抑制剂是可检测的正常狗的尿液中维持在不同的剂量摄入量从3至258毫克当量/天。在有和没有氟氢可的松的情况下进行观察。先前显示含有“尿毒症”抑制剂的相同Sephadex G-25凝胶过滤级分在分离的蟾蜍膀胱和大鼠生物测定系统中进行测试。来自狗的组分维持在258 meq qv-grafik ziel =“PIWIS 100488888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888888在相同的钠摄入量，而没有氟氢可的松的狗的分数也是利钠的（P小于0.01），但没有抑制SCC显着。相比之下，来自喂食含氟氢可的松的3 meq钠或不含氟氢可的松的91 meq钠的狗的级分在任一测定系统中均无显著影响。因此，在非尿毒症犬的尿液中发现了钠转运抑制剂。大鼠尿钠排泄的程度和蟾蜍膀胱SCC抑制的程度与犬的钠平衡状态相关。
The urine and serum of chronically uremic patients and dogs contain an inhibitor of sodium transport that reduces short-circuit current (SCC) in the toad bladder and produces natriuresis in the rat. The present studies represent an effort to determine whether the same inhibitor is detectable in urine of normal dogs maintained on a dosium intake varying from 3 to 258 meq/day. Observations were made with and without fludrocortisone. The same Sephadex G-25 gel filtration fraction previously shown to contain the "uremic" inhibitor was tested in both the isolated toad bladder and rat bioassay systems. The fraction from dogs maintained on 258 meq qodium plus 0.2 mg fludrocortisone/day consistently inhibited SCC in the toad bladder and induced a natriuresis in the rat (P less than 0.001). The fraction from dogs on the same sodium intake without fludrocortisone was also natriuretic (P less than 0.01) but did not inhibit SCC significantly. In contrast, the fraction from dogs fed 3 meq sodium with fludrocortisone or 91 meq sodium without fludrocortisone had no significant effect in either assay system. Thus, an inhibitor of sodium transport has been found in the urine of nonuremic dogs. Both the degree of natriuresis in the rat and the degree of inhibition of SCC in the toad bladder correlated with the state of sodium balance which ensued in the dog.