RIBONUCLEASE .5. STUDIES ON THE PROPERTIES AND DISTRIBUTION OF RIBONUCLEASE INHIBITOR IN THE RAT
RIBONUCLEASE .5. STUDIES ON THE PROPERTIES AND DISTRIBUTION OF RIBONUCLEASE INHIBITOR IN THE RAT
复制标题
DOI：
10.1016/0006-3002(56)90091-9
复制
发表时间：
1956-01-01
期刊：
影响因子：
--
通讯作者：
ROTH, JS
中科院分区：
文献类型：
--
作者：
ROTH, JS
研究了从大鼠肝匀浆制备的上清液中发现的RNA酶抑制剂的性质。该抑制剂对热不稳定且不可透析，对大鼠组织中的结晶性胰腺RNA酶和碱性RNA酶有活性，但对酸性RNA酶的影响很小。测定了从几种大鼠组织制备的上清液级分中抑制剂的分布。脑、肝脏和肺中的浓度最高，依次递减，而肾脏和肌肉中的浓度较低。大量的核糖核酸酶通常与抑制剂结合。结合的RNA酶等于或大于肝或肾的总线粒体碱性RNA酶活性。Pb ~（++）和对氯汞苯甲酸可使结合的酶释放，使抑制剂失活，但碘乙酸盐或碘乙酰胺不能使其失活。
The properties of a RNase inhibitor found in the supernatant fraction prepared from rat liver homogenates were investigated. The inhibitor, which is heat labile and non-dialyzable, is active against crystalline pancreatic RNase and alkaline RNases from rat tissues, but apparently has little effect on acid RNase. The distribution of inhibitor in the supernatant fractions prepared from several rat tissues was determined. Brain, liver and lung contain the highest concentrations in de-creasing order, while kidney and muscle contain smaller amounts. Large quantities of RNase are normally bound to the inhibitor. The bound RNase is equal to or greater than the total mitochondrial alkaline RNase activity of liver or kidney. The bound enzyme may be released and the inhibitor inactivated by Pb++ and p-chloro-mercuribenzoic acid but not by iodoacetate or iodoacetamide.