The L83L ORF of African swine fever virus strain Georgia encodes for a nonessential gene that interacts with the host protein IL-1β
The L83L ORF of African swine fever virus strain Georgia encodes for a nonessential gene that interacts with the host protein IL-1β
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DOI：
10.1016/j.virusres.2018.03.017
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发表时间：
2018-04-02
期刊：
影响因子：
5
通讯作者：
Gladue, Douglas P.
中科院分区：
文献类型：
--
作者：
Borca, Manuel V.;O'Donnell, Vivian;Gladue, Douglas P.
非洲猪瘟病毒（ASFV）引起猪的传染性和经常致命的疾病，对养猪业造成重大的经济后果。ASFV基因组编码150多个基因，但其中只有少数被详细研究过。在这里，我们报告了开放阅读框L83L的特征，该框架编码所有ASFV分离株中高度保守的蛋白质。构建了一种含有HA标记L83L蛋白的重组ASFV (ASFV- g -L83L-HA)，并用于证明L83L是一种瞬时表达的早期病毒蛋白。从高毒力的野地分离株Georgia2007 （ASFV- g）中获得了一株缺乏L83L基因的重组ASFV (ASFV- g - delta L83L)，并证明L83L是一个非必需基因。ASFV-G。与亲本病毒ASFV-G相比，Delta L83L在原代猪巨噬细胞中具有相似的复制。L83L的宿主蛋白相互作用分析鉴定IL-1 β为其宿主配体。对家猪的实验感染表明，ASFV-G- delta L83L的毒力与亲本病毒ASFV-G相同。
African swine fever virus (ASFV) causes a contagious and frequently lethal disease of pigs causing significant economic consequences to the swine industry. The ASFV genome encodes for more than 150 genes, but only a few of them have been studied in detail. Here we report the characterization of open reading frame L83L which encodes a highly conserved protein across all ASFV isolates. A recombinant ASFV harboring a HA tagged L83L protein was developed (ASFV-G-L83L-HA) and used to demonstrate that L83L is a transiently expressed early virus protein. A recombinant ASFV lacking the L83L gene (ASFV-G-Delta L83L) was developed from the highly virulent field isolate Georgia2007 (ASFV-G) and was used to show that L83L is a non-essential gene. ASFV-G.Delta L83L had similar replication in primary swine macrophage cells when compared to its parental virus ASFV-G. Analysis of host-protein interactions for L83L identified IL-1 beta as its host ligand. Experimental infection of domestic pigs showed that ASFV-G-Delta L83L is as virulent as the parental virus ASFV-G.