Immobilization of galactose ligands on thermoresponsive culture surface and its influence on cell adhesion/detachment.
Immobilization of galactose ligands on thermoresponsive culture surface and its influence on cell adhesion/detachment.
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DOI：
10.1016/j.jcis.2010.07.013
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发表时间：
2010-10
影响因子：
9.9
通讯作者：
Xiaoling He;Pingping Nie;Yankun Sun;Ying Wang;You-Yu Dong;Li Chen
中科院分区：
文献类型：
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作者：
Xiaoling He;Pingping Nie;Yankun Sun;Ying Wang;You-Yu Dong;Li Chen
通过紫外光引发N-异丙基丙烯酰胺（NIPAAm）与丙烯酸（AAc）共聚，并接枝到组织培养聚苯乙烯（TCPS）培养皿上，然后固定半乳糖配体，制备了一种新型的生物功能化温敏表面。结果表明，与NIPAAm共聚和半乳糖配体的固定化后，表面粗糙度增加。随着光接枝时间的延长，TCPS上接枝单体的量增加。随着TCPS表面接枝NIPAAm量的增加，TCPS表面的温度敏感性提高，羧基和半乳糖配体的引入增强了TCPS表面的亲水性，加速了TCPS表面的细胞脱落。研究了人肝癌细胞株HepG 2在改性表面上的粘附、增殖、脱落和转运。半乳糖配体的固定促进了细胞的粘附和增殖。HepG 2细胞生长在修饰的表面上，可以通过降低培养温度自发恢复。温度诱导法获得的细胞活力高于酶消化法。
A novel bio-functionalized thermoresponsive surface was prepared by UV-induced copolymerization of N-isopropylacrylamide (NIPAAm) and acrylic acid (AAc) and grafting onto tissue culture polystyrene (TCPS) dishes, followed by immobilization of galactose ligands. The results indicated that the roughness of surfaces was increased after copolymerization with NIPAAm and immobilization of galactose ligands. The amount of monomers grafted on TCPS was increased with increasing photografting time. The temperature sensitivity of surface was improved with increasing amount of NIPAAm grafted on TCPS, and surface hydrophilicity was enhanced by the introduction of carboxyl groups and galactose ligands, which accelerated cell detachment. Adhesion, proliferation, detachment and transshipment of human hepatoma cell line (HepG2) on the modified surfaces were examined. The immobilization of galactose ligands facilitated the cell adhesion and proliferation. HepG2 cells growing on the modified surfaces could be recovered spontaneously by only reducing culture temperature. The activity of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.