Phenanthrene degradation in soil using biochar hybrid modified bio-microcapsules: Determining the mechanism of action via comparative metagenomic analysis
Phenanthrene degradation in soil using biochar hybrid modified bio-microcapsules: Determining the mechanism of action via comparative metagenomic analysis
复制标题
使用生物炭杂化改性生物微胶囊降解土壤中的菲：通过比较宏基因组分析确定作用机制
DOI：
10.1016/j.scitotenv.2021.145798
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发表时间：
2021-02-19
影响因子：
9.8
通讯作者：
Dang, Zhi
中科院分区：
文献类型：
--
作者：
Deng, Fucai;Dou, Rongni;Dang, Zhi
采用生物炭（BC）杂化改性技术，以提高层层组装（LBL）微胶囊固定化降解菌对菲（PHE）污染土壤的修复效果。采用分类学和功能宏基因组学方法研究了菲污染土壤生物修复过程中微生物群落结构和功能基因组成的变化。使用BC（3%）混合LBL生物微胶囊在土壤中进行初始PHE浓度为100 mg kg（-1）干土的生物强化（BC-LBL，2.0 g kg（-1）干土，107个菌落形成单位细胞g（-1）干土）生长速度快; LBL降解剂对苯丙氨酸的降解率为66.2%，而LBL降解剂对苯丙氨酸的降解率为80.5%，高于LBL。在BC-LBL处理中，鞘氨醇单胞菌属、链霉菌属、Gemmatirosa、Ramlibacter、黄固杆菌属、Phycococcus、小单孢菌属、酸杆菌属、分枝杆菌属和芽单胞菌属的数量明显多于LBL处理。功能基因注释结果表明，BC-LBL处理的基因数目比LBL处理的多。前者功能更丰富，主要与细菌的生长、繁殖、代谢和运输有关。BC杂交促进微囊化细菌降解PHE的原因可能是BC具有较强的吸附性，富集了细菌生长繁殖所需的营养物质，增强了PHE对BC-LBL的传质性能;同时，BC还能刺激和改善降解菌的代谢和膜运输，最终提高降解功能。(c)2021爱思唯尔有限公司版权所有。
A strategy involving biochar (BC) hybrid modification was developed to promote the bioremediation effect of degrading bacteria immobilized in layer-by-layer assembly (LBL) microcapsules for the treatment of phenan-threne (PHE) polluted soil. A taxonomic and functional metagenomic approach was used to investigate changes in the microbial community structures and functional gene compositions in the PHE-polluted soil during the bio-remediation process. Biofortification with an initial PHE concentration of 100 mg kg(-1) dry soil in soils using the BC (3%) hybrid LBL bio-microcapsule (BC-LBL, 2.0 g kg(-1) dry soil, 107 colony forming unite cell g(-1) dry soil) was faster; further, a higher PHE degradation efficiency (80.5% after 25 d) was achieved when compared with that by the LBL agent (66.2% after 25 d) used. Sphingomonas, Streptomyces, Gemmatirosa, Ramlibacter, Flavisolibacter, Phycicoccus, Micromonospora, Acidobacter, Mycobacterium and Gemmatimonas were more abundant in BC-LBL treatment than those in LBL one. Functional gene annotation results showed that more gene number with BC-LBL treatment than those with LBL one. More abundant functions in the former were primarily related to the growth, reproduction, metabolism, and transportation of bacteria. BC hybridization promoting PHE degradation by microencapsulated bacteria may be due to the strong adsorption property of BC, which results in the enrichment of the nutrients that needed for bacterial growth and reproduction, as well as enhancing the mass transfer performance of PHE to BC-LBL; Meanwhile, BC could also stimulate and improve the metabolism and membrane transportation of the degrading bacteria, and finally improving the degradation function. (c) 2021 Elsevier B.V. All rights reserved.