Ultraviolet Radiant Energy-Dependent Functionalization Regulates Cellular Behavior on Titanium Dioxide Nanodots
Ultraviolet Radiant Energy-Dependent Functionalization Regulates Cellular Behavior on Titanium Dioxide Nanodots
复制标题
紫外线辐射能量依赖性功能化调节二氧化钛纳米点上的细胞行为。
DOI：
10.1021/acsami.0c07761
复制
发表时间：
2020
影响因子：
9.5
通讯作者：
Wang Huiming
中科院分区：
文献类型：
--
作者：
Liu Chao;Sun Mouyuan;Wang Yu;Zhu Tianer;Ye Guanchen;You Dongqi;Dong Lingqing;Zhao Wenquan;Cheng Kui;Weng Wenjian;Zhang Yu Shrike;Yu Mengfei;Wang Huiming
二氧化钛（TiO 2）光功能化已被证明是一种有效的表面改性方法，用于种植体的骨整合。然而，对光功能化的机制认识不足，限制了其临床应用。在这里，我们报告了一个紫外（UV）辐射能量依赖的功能化TiO 2纳米点（TN）表面。我们发现随着紫外线辐射能量（URE）的积累，细胞的粘附、增殖和成骨分化逐渐增强。最佳的功能化处理能量被发现是2000 mJ/cm 2，这可以调节TN表面上的细胞特异性行为。增强的细胞行为是通过细胞外基质（ECM）蛋白的吸附和功能位点暴露来调节的，这是由URE引起的表面物理化学变化的结果。URE和表面羟基的重建之间的相关性被认为是这种能量依赖性功能化的替代机制。我们还证明了FAK-RHOA和ERK 1/2信号通路在介导URE依赖性细胞行为方面的协同作用。总的来说，这项研究提供了一个新的见解，光功能化的机制，指导种植体的设计和光功能化的临床实践。
Titanium dioxide (TiO2) photofunctionalization has been demonstrated as an effective surface modification method for the osseointegration of implants. However, the insufficient understanding of the mechanism underlying photofunctionalization limits its clinical applications. Here, we report an ultraviolet (UV) radiant energy-dependent functionalization on TiO2nanodots (TN) surfaces. We found the cell adhesion, proliferation, and osteogenic differentiation gradually increased with the accumulation of UV radiant energy (URE). The optimal functionalizing treatment energy was found to be 2000 mJ/cm2, which could regulate cell-specific behaviors on TN surfaces. The enhanced cell behaviors were regulated by the adsorption and functional site exposure of the extracellular matrix (ECM) proteins, which were the result of the surface physicochemical changes induced by the URE. The correlation between the URE and the reconstruction of surface hydroxyl groups was considered as an alternative mechanism of this energy-dependent functionalization. We also demonstrated the synergistic effects of FAK-RHOA and ERK1/2 signaling pathways on mediating the URE-dependent cell behaviors. Overall, this study provides a novel insight into the mechanisms of photofunctionalization, guiding the design of implants and the clinical practice of photofunctionalization.