Cationic liposomes for gene delivery.
Cationic liposomes for gene delivery.
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DOI：
10.1517/17425247.2.2.237
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发表时间：
2005-03-01
影响因子：
6.6
通讯作者：
de Lima, Maria Pedroso
中科院分区：
文献类型：
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作者：
Simoes, Sergio;Filipe, Ana;de Lima, Maria Pedroso
阳离子脂质体-DNA复合体(Lipoplex)构成了一种潜在可行的替代病毒载体来传递治疗基因的方法。这篇综述将集中于控制脂复合体生物活性的各种参数，从它们的形成方式到体内行为。特别强调脂复合体与细胞的相互作用机制，试图剖析需要跨越的不同障碍才能有效地进行基因表达。将涉及旨在克服其某些局限性的脂基基因传递系统的制定的新趋势。最后，举例说明阳离子脂质体在临床应用中的潜力。
Cationic liposome-DNA complexes (lipoplexes) constitute a potentially viable alternative to viral vectors for the delivery of therapeutic genes. This review will focus on various parameters governing lipoplex biological activity, from their mode of formation to in vivo behaviour. Particular emphasis is given to the mechanism of interaction of lipoplexes with cells, in an attempt to dissect the different barriers that need to be surpassed for efficient gene expression to occur. Aspects related to new trends in the formulation of lipid-based gene delivery systems aiming at overcoming some of their limitations will be covered. Finally, examples illustrating the potential of cationic liposomes in clinical applications will be provided.