Centromeric Transcription Regulates Aurora-B Localization and Activation.
Centromeric Transcription Regulates Aurora-B Localization and Activation.
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DOI：
10.1016/j.celrep.2016.04.054
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发表时间：
2016-05-24
期刊：
影响因子：
8.8
通讯作者：
Blower MD
中科院分区：
文献类型：
--
作者：
Blower MD
Centromeric transcription is widely conserved, however it is not clear what role centromere transcription plays during mitosis. Here I find that centromeres are transcribed in Xenopus egg extracts into a long noncoding RNA (lncRNA; cen-RNA) that localizes to mitotic centromeres, chromatin, and spindles. Cen-RNAs bind to the Chromosomal Passenger Complex (CPC) in vitro and in vivo. Blocking transcription or antisense inhibition of cen-RNA leads to a reduction of CPC localization to the inner centromere and misregulation of CPC component Aurora-B activation, independently of known centromere recruitment pathways. Additionally, transcription is required for normal bipolar attachment of kinetochores to the mitotic spindle, consistent with a role for cen-RNA in CPC regulation. This work demonstrates that cen-RNAs promote normal kinetochore function through regulation of the localization and activation of the CPC and confirm that lncRNAs are components of the centromere.
