HSPRO Controls Early Nicotiana attenuata Seedling Growth during Interaction with the Fungus Piriformospora indica1[C][W][OA]
HSPRO Controls Early Nicotiana attenuata Seedling Growth during Interaction with the Fungus Piriformospora indica1[C][W][OA]
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DOI：
10.1104/pp.112.203976
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发表时间：
2012-08
期刊：
影响因子：
7.4
通讯作者：
Stefan Schuck;Iris Camehl;Paola A Gilardoni;Ralf Oelmueller;I. Baldwin;G. Bonaventure
中科院分区：
文献类型：
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作者：
Stefan Schuck;Iris Camehl;Paola A Gilardoni;Ralf Oelmueller;I. Baldwin;G. Bonaventure
在先前的旨在鉴定烟草对咀嚼昆虫的反应的调节剂的研究中，发现在模拟食草动物后强烈诱导匹配HSPRO（糖甜菜Hs 1 pro-1的正向同源物）基因的26-核苷酸标签（Gilardoni et al.，2010年）。在此，我们描述了HSPRO在转基因N. envelopata植物沉默其表达（IR-HSPRO）。在野生型植物中，HSPRO的表达不仅诱导在模拟植食动物，但也当叶接种假单胞菌番茄DC 3000和根与生长促进真菌梨形孢印度。HSPRO表达的减少并不影响对烟草天蛾或番茄疫霉致病变种DC 3000感染率的直接防御的调节。然而，减少HSPRO表达积极影响早期幼苗生长过程中的相互作用与P.印度;真菌定殖IR-HSPRO幼苗增加了30%的鲜生物量相比，野生型。嫁接实验表明，减少HSPRO在根中的表达是足以诱导差异生长促进在根和芽。这种影响伴随着417个基因在定殖根中的表达变化，其中大部分是代谢基因。ir-hspro和野生型定殖根的代谢谱（通过液相色谱-飞行时间质谱分析）没有重大差异，这表明参与了加速的代谢速率。我们的结论是，HSPRO参与了整个植物的生长生理变化时，幼苗与P.印度。
In a previous study aimed at identifying regulators of Nicotiana attenuata responses against chewing insects, a 26-nucleotide tag matching the HSPRO (ORTHOLOG OF SUGAR BEET Hs1pro-1) gene was found to be strongly induced after simulated herbivory (Gilardoni et al., 2010). Here we characterized the function of HSPRO during biotic interactions in transgenic N. attenuata plants silenced in its expression (ir-hspro). In wild-type plants, HSPRO expression was not only induced during simulated herbivory but also when leaves were inoculated with Pseudomonas syringae pv tomato DC3000 and roots with the growth-promoting fungus Piriformospora indica. Reduced HSPRO expression did not affect the regulation of direct defenses against Manduca sexta herbivory or P. syringae pv tomato DC3000 infection rates. However, reduced HSPRO expression positively influenced early seedling growth during interaction with P. indica; fungus-colonized ir-hspro seedlings increased their fresh biomass by 30% compared with the wild type. Grafting experiments demonstrated that reduced HSPRO expression in roots was sufficient to induce differential growth promotion in both roots and shoots. This effect was accompanied by changes in the expression of 417 genes in colonized roots, most of which were metabolic genes. The lack of major differences in the metabolic profiles of ir-hspro and wild-type colonized roots (as analyzed by liquid chromatography time-of-flight mass spectrometry) suggested that accelerated metabolic rates were involved. We conclude that HSPRO participates in a whole-plant change in growth physiology when seedlings interact with P. indica.