Experimental metastasis is suppressed in MMP-9-deficient mice
Experimental metastasis is suppressed in MMP-9-deficient mice
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DOI：
10.1023/a:1006603723759
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发表时间：
1999-03-01
影响因子：
4
通讯作者：
Uehira, M
中科院分区：
文献类型：
--
作者：
Itoh, T;Tanioka, M;Uehira, M
基质金属蛋白酶（MMPs）被认为在肿瘤侵袭和转移中起关键作用。MMP-9（明胶酶B）在肿瘤转移中的作用在通过使用胚胎干细胞的基因靶向产生的MMP-9缺陷小鼠中进行了检查。MMP-9缺陷型小鼠发育正常，具有生育能力。在这些小鼠中，静脉内植入的B16-BL 6黑色素瘤细胞或刘易斯肺癌细胞的转移性集落数量对于B16-BL 6黑色素瘤下降了45%，对于刘易斯肺癌下降了59%（分别为p=0.03和p=0.0043）。明胶酶谱分析表明，两种肿瘤细胞系本身不分泌MMP-9，但肿瘤细胞周围的宿主细胞在体内分泌MMP-9。这些结果表明，宿主来源的MMP-9在肿瘤转移过程中起重要作用。
Matrix metalloproteinases (MMPs) are thought to play a key role in tumor invasion and metastasis. The role of MMP-9 (gelatinase B) in tumor metastasis was examined in MMP-9-deficient mice produced by gene targeting using embryonic stem cells. MMP-9-deficient mice develop normally and are fertile. In these mice, the number of metastatic colonies of B16-BL6 melanoma cells or Lewis lung carcinoma cells that were implanted intravenously fell by 45% for B16-BL6 melanoma and 59% for Lewis lung carcinoma (p=0.03 and p=0.0043, respectively). Gelatin zymography showed that both tumor cell lines did not secrete MMP-9 by themselves but the host cells surrounding the tumor cells secrete MMP-9 in vivo. These results indicated that host-derived MMP-9 plays an important role in the process of tumor metastasis.