Diversity in expression of phosphorus (P) responsive genes in Cucumis melo L.
Diversity in expression of phosphorus (P) responsive genes in Cucumis melo L.
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DOI：
10.1371/journal.pone.0035387
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hammond JP
中科院分区：
文献类型：
--
作者：
Fita A;Bowen HC;Hayden RM;Nuez F;Picó B;Hammond JP
在许多土壤中，磷是植物生长的主要限制养分。对模式物种的研究已经确定了参与植物适应低土壤磷有效性的基因。然而，关于蔬菜作物这些适应性的遗传基础的信息很少。在这方面，甜瓜的序列数据现在使得鉴定候选磷响应基因的甜瓜同源物成为可能，这些基因的表达可以用来解释最近在该物种中观察到的根系对低磷有效性适应的多样性。通过比较8个候选基因（Cm-PAP10.1、Cm-PAP10.2、Cm-RNS1、Cm-PPCK1、cm -转移酶、Cm-SQD1、Cm-DGD1和Cm-SPX2）在缺磷和缺磷条件下的表达，研究了9个不同甜瓜材料对缺磷的转录响应。观察了不同甜瓜品种对磷饥饿的响应，包括植株形态、磷吸收、磷利用效率（PUE）和基因表达的差异。所有研究基因在磷饥饿条件下均上调表达。缺磷条件下参与磷动员和再动员的基因Cm-PAP10.1、Cm-PAP10.2和Cm-RNS1的表达差异可以部分解释不同甜瓜对磷吸收和PUE的差异。其他基因的表达水平在甜瓜品种间存在差异，但对表型响应的贡献较小。这是这些基因首次在甜瓜磷饥饿反应的背景下被描述。不同甜瓜种质间基因表达水平和磷利用效率存在显著差异，基因表达水平与表型指标存在显著相关。
Phosphorus (P) is a major limiting nutrient for plant growth in many soils. Studies in model species have identified genes involved in plant adaptations to low soil P availability. However, little information is available on the genetic bases of these adaptations in vegetable crops. In this respect, sequence data for melon now makes it possible to identify melon orthologues of candidate P responsive genes, and the expression of these genes can be used to explain the diversity in the root system adaptation to low P availability, recently observed in this species. Transcriptional responses to P starvation were studied in nine diverse melon accessions by comparing the expression of eight candidate genes (Cm-PAP10.1, Cm-PAP10.2, Cm-RNS1, Cm-PPCK1, Cm-transferase, Cm-SQD1, Cm-DGD1 and Cm-SPX2) under P replete and P starved conditions. Differences among melon accessions were observed in response to P starvation, including differences in plant morphology, P uptake, P use efficiency (PUE) and gene expression. All studied genes were up regulated under P starvation conditions. Differences in the expression of genes involved in P mobilization and remobilization (Cm-PAP10.1, Cm-PAP10.2 and Cm-RNS1) under P starvation conditions explained part of the differences in P uptake and PUE among melon accessions. The levels of expression of the other studied genes were diverse among melon accessions, but contributed less to the phenotypical response of the accessions. This is the first time that these genes have been described in the context of P starvation responses in melon. There exists significant diversity in gene expression levels and P use efficiency among melon accessions as well as significant correlations between gene expression levels and phenotypical measurements.
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影响因子：
11.6
作者：
Hamburger, D;Rezzonico, E;Poirier, Y
通讯作者：
Poirier, Y
DOI：
10.1073/pnas.95.4.1950
发表时间：
1998-02-17
影响因子：
11.1
作者：
Essigmann, B;Güler, S;Benning, C
通讯作者：
Benning, C
影响因子：
4.1
作者：
Chen, Zhi-Hui;Nimmo, Gillian A.;Nimmo, Hugh G.
通讯作者：
Nimmo, Hugh G.
影响因子：
6.9
作者：
Hammond, John P.;Broadley, Martin R.;Greenwood, Duncan J.
通讯作者：
Greenwood, Duncan J.
影响因子：
6.9
作者：
Hammond, John P.;White, Philip J.
通讯作者：
White, Philip J.