Radiation-induced changes in the cell membrane of cultured human endothelial cells.
Radiation-induced changes in the cell membrane of cultured human endothelial cells.
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辐射引起的培养人内皮细胞细胞膜的变化。
DOI：
10.2307/3576593
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发表时间：
1985
影响因子：
3.4
通讯作者：
A. Boon
中科院分区：
文献类型：
--
作者：
G. B. Vegt;A. Wassenaar;E. W. K. Kawilarang;P. Schütte;M. Van der Linden;M. Di Bon;A. Boon
我们研究了辐射对氨基酸和葡萄糖转运动力学特性的影响，以及对细胞膜结合酶5‘-核苷酸酶活性的影响，以及前列腺素E1对受体介导的环磷酸腺苷合成的影响。辐射通过减少氨基酸与转运单位的结合来抑制钠依赖的氨基酸转运。葡萄糖的转运似乎是一个不依赖于钠的过程，但最大转运速度的增加暂时刺激了葡萄糖的转运。未发现对与运输单元的结合有影响。照射后48h，照射后48h，5‘-核苷酸酶活性升高，前列腺素E_1刺激的环磷酸腺苷合成减少。结论是，辐射通过损伤转运单位来减少钠依赖的转运，不损害钠非依赖的过程，而对膜结合酶活性和受体介导的过程有相反的影响。
We investigated the effect of irradiation on the kinetic characteristics of amino acid and glucose transport, and the effect on the activity of the cell membrane-bound enzyme 5'-nucleotidase and on the receptor-mediated stimulation of cyclic adenosine monophosphate synthesis by prostaglandin E1. Irradiation inhibited the sodium-dependent amino acid transport by a reduced binding of the amino acid to the transport unit. The transport of glucose, which appeared to be a sodium-independent process, was temporarily stimulated by increased maximal velocity of the transport. No effect was found on the binding to the transport unit. Irradiation increased the 5'-nucleotidase activity and decreased the prostaglandin E1-stimulated cyclic adenosine monophosphate synthesis 48 h after exposure to 20 Gy. It is concluded that irradiation decreases sodium-dependent transport by impairment of the transport unit, does not impair a sodium-independent process, and has opposite effects on membrane-bound enzyme activity and a receptor-mediated process.
急性辐射对培养的主动脉内皮细胞中纤溶酶原激活剂活性的含量和释放的影响。
影响因子：
3.4
作者：
Ts'ao,C;Ward,WF
通讯作者：
Ward,WF