Secretory leukocyte protease inhibitor expression in various types of gastritis: a specific role of Helicobacter pylori infection
Secretory leukocyte protease inhibitor expression in various types of gastritis: a specific role of Helicobacter pylori infection
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分泌性白细胞蛋白酶抑制剂在各类胃炎中的表达：幽门螺杆菌感染的特定作用
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
P. Malfertheiner
中科院分区：
文献类型：
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作者：
I. Hritz;D. Kuester;M. Vieth;L. Herszényi;M. Stolte;A. Roessner;Z. Tulassay;T. Wex;P. Malfertheiner
目的分泌性白细胞蛋白酶抑制剂（SLPI）是一种多功能的胃肠道黏膜蛋白，具有抗菌、抗炎等作用。SLPI表达通常在炎症过程中被诱导，然而，幽门螺杆菌介导的胃炎与胃窦SLPI水平显著降低相关。本研究的目的是研究胃粘膜SLPI的下调是否代表了H。幽门螺杆菌感染或通常与胃炎症有关。方法采用免疫组化方法对85例石蜡包埋的大肠癌组织中SLPI的表达进行回顾性分析。幽门螺杆菌感染13例，非甾体抗炎药（NSAID）增强型18例，自身免疫性11例，淋巴细胞性胃炎26例，H.幽门阴性对照（n=17）。使用免疫反应性评分对染色强度进行半定量分析。使用方差分析检验进行差异的统计学分析。结果与对照组相比，H.幽门介导性胃炎和淋巴细胞性胃炎显著降低（P<0.001），而上皮SLPI表达在胃窦或胃体的NSAID增强性胃炎和自身免疫性胃炎中分别不受影响。H.幽门螺杆菌感染组和淋巴细胞性胃炎组浸润的免疫细胞SLPI表达明显降低（P<0.01），而自身免疫性胃炎组浸润的免疫细胞SLPI表达明显高于其他各组（P<0.03）。结论胃窦黏膜SLPI表达下调与H.幽门螺杆菌感染并不是一般的胃炎症现象。
Objectives Secretory leukocyte protease inhibitor (SLPI) represents a multifunctional protein of the gastrointestinal mucosa exerting antimicrobial and anti-inflammatory effects. SLPI expression is generally induced during inflammation; however, Helicobacter pylori-meditated gastritis is associated with significantly decreased antral SLPI levels. The aim of the study was to investigate whether SLPI downregulation of gastric mucosa represents a specific phenomenon of H. pylori infection or is generally linked to gastric inflammation. Methods SLPI expression was retrospectively analysed by immunohistochemistry in 85 paraffin-embedded samples: H. pylori-induced (n=13), non-steroidal anti-inflammatory drug (NSAID)-enhanced (n=18), autoimmune (n=11), lymphocytic gastritis (n=26) and H. pylori-negative controls (n=17). The intensity of the staining was semiquantitatively analysed using an immunoreactivity score. Statistical analysis of differences was performed using an analysis of variance test. Results In comparison with the control group, the SLPI expression of antral mucosa in H. pylori-mediated and lymphocytic gastritis was significantly lower (P<0.001), whereas epithelial SLPI expression was not affected in NSAID-enhanced and autoimmune gastritis either in the antrum or corpus, respectively. Both the H. pylori-mediated and lymphocytic gastritis revealed a significantly lower expression of SLPI in infiltrating immune cells (P<0.01), whereas immune cells infiltrating the corpus in autoimmune gastritis showed higher SLPI levels than the immune cells of other groups (P<0.03). Conclusion The local downregulation of SLPI in antral mucosa is specifically linked to H. pylori infection and is not a general phenomenon of gastric inflammation.
影响因子：
15.9
作者：
MCNEELY, TB;DEALY, M;WAHL, SM
通讯作者：
WAHL, SM
影响因子：
158.5
作者：
PARSONNET, J;FRIEDMAN, GD;SIBLEY, RK
通讯作者：
SIBLEY, RK