Possible involvement of nerve growth factor in dysmenorrhea and dyspareunia associated with endometriosis.
Possible involvement of nerve growth factor in dysmenorrhea and dyspareunia associated with endometriosis.
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DOI：
10.1507/endocrj.ej13-0027
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发表时间：
2013-11
影响因子：
2
通讯作者：
Takashi Kajitani;T. Maruyama;H. Asada;H. Uchida;H. Oda;Sayaka Uchida;K. Miyazaki;T. Arase;
中科院分区：
文献类型：
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作者：
Takashi Kajitani;T. Maruyama;H. Asada;H. Uchida;H. Oda;Sayaka Uchida;K. Miyazaki;T. Arase;
神经生长因子(NGF)最近被认为是促进神经纤维生长的关键因素之一，也是多种疾病中疼痛的发生和维持的关键因素之一。本研究的目的是探讨神经生长因子在子宫内膜异位症相关盆腔疼痛中的作用。组织和腹腔液样本来自95名经腹腔镜和组织病理证实的子宫内膜异位症患者和59名非子宫内膜异位症的对照女性。用实时定量RT-PCR和免疫组织化学方法分别检测NGF基因和蛋白的表达水平。采用双抗体夹心法测定腹腔液中神经生长因子(PF-NGF)的浓度。采用言语评定量表评定月经困难和痛经程度。实时荧光定量RT-PCR分析显示，卵巢子宫内膜异位症和腹膜子宫内膜异位症组织中NGF基因的表达显著高于正常子宫内膜组织。免疫组织化学分析表明，NGF在卵巢子宫内膜异位症和腹膜子宫内膜异位症的腺体中有明显的表达，且主要分布在腺体内，而在正常子宫内膜中仅有微弱表达。虽然Pf-NGF在一些正常人和子宫内膜异位症患者中检测不到，但腹腔液中Pf-NGF在有重度疼痛的子宫内膜异位症患者中比在疼痛较轻的患者中更常见。我们的结果提示，腹膜腔局部产生的神经生长因子可能参与了子宫内膜异位症相关盆腔疼痛的发生。
Nerve growth factor (NGF) has been recently proposed as one of the key factors responsible not only for promotion of nerve fiber growth but also for the onset and maintenance of pain in a variety of diseases. The aim of this study was to investigate the role of NGF in the pelvic pain associated with endometriosis. Tissue and peritoneal fluid samples were collected from 95 women with laparoscopically and histopathologically confirmed endometriosis and 59 control women without endometriosis. Expression levels of NGF mRNA and protein were examined using real-time RT-PCR and immunohistochemistry, respectively. Concentration of NGF in the peritoneal fluid (PF-NGF) was measured using ELISA. The degree of dyspareunia and dysmenorrhea was evaluated using a verbal rating scale. Real-time RT-PCR analysis revealed that NGF mRNA was significantly more abundant in the ovarian endometriomas and peritoneal endometriosis than in the normal control endometrium. Immunohistochemical analyses demonstrated that NGF was prominently expressed and preferentially localized to the glands of the ovarian endometriomas and peritoneal endometriosis, whereas it was only weakly detectable in the normal endometrium. Although PF-NGF was undetectable in some normal subjects and endometriosis patients, elevated PF-NGF in the peritoneal fluid was more frequently observed in endometriosis patients with severe pain than in those with less severe pain. Our results suggest that NGF produced locally in the peritoneal cavity may be involved in the generation of endometriosis-associated pelvic pain.