[Effects of electroacupuncture on BNDF/mTORC1 signaling pathway and synaptic plasticity in prefrontal cortex of rats exposed to chronic unpredictable mild stress].
[Effects of electroacupuncture on BNDF/mTORC1 signaling pathway and synaptic plasticity in prefrontal cortex of rats exposed to chronic unpredictable mild stress].
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DOI：
10.13702/j.1000-0607.201293
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发表时间：
2022-01-25
期刊：
影响因子：
--
通讯作者：
Fu, Wen-Bin
中科院分区：
文献类型：
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作者：
Gao, Jing;Lai, Ming-Yin;Fu, Wen-Bin
目的：探讨电针对抑郁模型大鼠前额叶皮质（PFC）脑源性神经营养因子（BDNF）/哺乳动物雷帕霉素靶蛋白复合物1（mTORC 1）信号通路相关蛋白和突触相关蛋白表达及树突棘密度的影响。揭示电针调节突触可塑性改善抑郁症状的机制。将36只健康雄性SD大鼠随机分为正常组、模型组、电针组和东莨菪碱组，每组9只。采用慢性不可预见性轻度应激（CIMs）结合隔离饲养建立大鼠抑郁模型。电针组在“百会”、“印堂”、“合谷”、“太冲”等穴位电针（2 Hz/100 Hz，1- 1.2mA），每次20 min，每日1次，连续14 d;采用蔗糖偏好实验（SPT）和新颖性抑制摄食实验观察各组大鼠的蔗糖偏好和摄食潜伏期。Western blot检测BDNF/mTORC 1信号通路蛋白及突触相关蛋白PSD 95、Synapsin Ⅰ和GluR 1的表达水平。Golgi-Cox染色观察PFC内第Ⅴ层锥体神经元顶树突上树突棘的总密度以及成熟、未成熟和丝状足样树突棘的密度。结果：与正常组相比，模型组大鼠对蔗糖的偏好性明显降低（P
OBJECTIVE: To investigate the effects of electroacupuncture (EA) on the expression of related proteins in the brain-derived neurotrophic factor (BDNF)/mammalian target of rapamycin complex 1 (mTORC1) signaling pathway and synapse-associated proteins and the density of dendrite spines in the prefrontal cortex (PFC) of depression model rats, and to reveal the underlying mechanism by which EA regulates the synaptic plasticity to improve depressive symptoms.METHODS: Thirty-six healthy male Sprague-Dawley (SD) rats were randomly divided into normal group, model group, EA group, and scopolamine (SCOP) group, with 9 in each group. The depression model was established by exposing rats to chronic unpredictable mild stress (CUMS) combined with isolated feeding. Rats in the EA group were treated with EA (2 Hz/100 Hz, 1-1.2 mA) at "Baihui" (GV20), "Yintang" (EX-HN3), "Hegu" (LI4), and "Taichong" (LR3), 20 min each time, once per day, for 14 d, while those in the SCOP group treated with intraperitoneal injection of 25 mug/kg SCOP, once every 16 h, for 14 d. The sucrose preference and feeding latency of rats in each group were observed in the sucrose preference test (SPT) and novelty-suppressed feeding test. The expression levels of proteins in the BDNF/mTORC1 signaling pathway and synapse-associated proteins PSD95, Synapsin Ⅰ, and GluR1 were assayed by Western blot. Golgi-Cox staining was conducted for exploring the total density of dendritic spines on the apical dendrites of layer Ⅴ pyramidal neurons in PFC as well as the densities of mature, immature, and filopodial-like dendritic spines.RESULTS: Compared with the normal group, the model group exhibited significantly decreased sucrose preference (P