Evaluation of human FcγRIIA (CD32) and FcγRIIIB (CD16) polymorphisms in Caucasians and African-Americans using salivary DNA
Evaluation of human FcγRIIA (CD32) and FcγRIIIB (CD16) polymorphisms in Caucasians and African-Americans using salivary DNA
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DOI：
10.1128/cdli.7.4.676-681.2000
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发表时间：
2000-07-01
期刊：
影响因子：
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通讯作者：
Wilson, ME
中科院分区：
文献类型：
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作者：
van Schie, RCAA;Wilson, ME
免疫球蛋白G（IgG）的Fc区的Tno类低亲和力受体（Fc γ R）在静息的人嗜中性粒细胞上组成型表达。这些受体，称为Fc γ RIIa（CD 32）和Fc γ RIIIb（CD 16），显示出双等位基因多态性，其具有关于结合和/或摄取由人IgG亚类抗体调理的靶标的功能后果。CD 32的H131-R131多态性影响人IgG 2与中性粒细胞的结合，并在较小程度上影响人IgG 3与中性粒细胞的结合。CD 16的中性粒细胞抗原（NA 1-NA 2）多态性影响由人IgG 1和IgG 3调理的细菌的吞噬效率。这些多态性可能影响宿主对某些感染性和/或自身免疫性疾病的易感性，促使人们对开发用于在各种临床环境中确定CD 32和CD 16基因型的简便方法感兴趣，我们以前报道过，在基于PCR的CD 32和CD 16多态性分析中，来自唾液的基因组DNA是血液DNA的合适替代物。我们第一次利用这种基于唾液DNA的方法来确定271名高加索人和118名非洲人的CD 32和CD 16基因型，美国受试者，并调查某些CD 32和CD 16基因型在这两个种族中可能的连锁不平衡，H131和R131基因频率分别为0.45和0.55，在白种人中为0.59，在非裔美国人中为0.59。NA 1和NA 2基因频率在白人中分别为0.38和0.62，在非裔美国人中分别为0.39和0.61。由于Fc γ RIIa和Fc γ RIIIb协同触发中性粒细胞，我们还评估了这两组中不同CD 32和CD 16基因型组合的频率。在这两组中，R/R131-NA 2/NA 2基因型组合比H/H131-NA 1/NA 1组合更常见（白人为3倍，非洲裔美国人为7倍）。具有组合的R/R131-NA 2/NA 2基因型的个体是否处于发生感染性和/或自身免疫性疾病的更大风险中需要进一步调查，这可以方便地使用来自唾液而不是血液的DNA进行。
Tno classes of low-affinity receptors for the Fc region of immunoglobulin G (IgG) (Fc gamma R) are constitutively expressed on resting human neutrophils, These receptors, termed Fc gamma RIIa (CD32) and Fc gamma RIIIb (CD16), display biallelic polymorphisms which have functional consequences with respect to binding and/or ingestion of targets opsonized by human IgG subclass antibodies. The H131-R131 polymorphism of CD32 influences binding of human IgG2 and, to a lesser extent, human IgG3 to neutrophils. The neutrophil antigen (NA1-NA2) polymorphism of CD16 influences the efficiency of phagocytosis of bacteria opsonized by human IgG1 and IgG3, These polymorphisms may influence host susceptibility to certain infectious and/or autoimmune diseases, prompting interest in the development of facile methods for determination of CD32 and CD16 genotype in various clinical settings, We previously reported that genomic DNA from saliva is a suitable alternative to DNA from blood in PCR-based analyses of CD32 and CD16 polymorphisms, In the present study, we utilized for the first time this salivary DNA-based methodology to define CD32 and CD16 genotypes in 271 Caucasian and 118 African-American subjects and to investigate possible linkage disequilibrium between certain CD32 and CD16 genotypes in these two ethnic groups, H131 and R131 gene frequencies were 0.45 and 0.55, respectively, among Caucasians and 0.59 among African-Americans. NA1 and NA2 gene frequencies were 0.38 and 0.62 among Caucasians and 0.39 and 0.61 among African-Americans. Since Fc gamma RIIa and Fc gamma RIIIb synergize in triggering neutrophils, we also assessed the frequency of different CD32 and CD16 genotype combinations in these two groups. In both groups, the R/R131-NA2/NA2 genotype combination was more common than the H/H131-NA1/NA1 combination (threefold for Caucasians versus sevenfold for African-Americans). Whether individuals with the combined R/R131-NA2/NA2 genotype are at greater risk for development of infectious and/or autoimmune diseases requires further investigation, which can he conveniently performed using DNA from saliva rather than blood.