Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.
Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.
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DOI：
10.1007/978-1-4939-2874-3_7
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Crispin M
中科院分区：
文献类型：
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作者：
Bitto D;Harvey DJ;Halldorsson S;Doores KJ;Pritchard LK;Huiskonen JT;Bowden TA;Crispin M
由于糖蛋白分离的困难和低样品丰度，病毒体衍生的糖蛋白的聚糖分析是具有挑战性的。在这里，我们描述了如何离子迁移质谱法可以用来获得病毒体样品的光谱。我们还描述了如何负离子的聚糖片段可以用来探测病毒体聚糖的结构特征。
Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.