The RNA world of the nucleolus: Two major families of small RNAs defined by different box elements with related functions
The RNA world of the nucleolus: Two major families of small RNAs defined by different box elements with related functions
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DOI：
10.1016/s0092-8674(00)80156-7
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发表时间：
1996-09-06
期刊：
影响因子：
64.5
通讯作者：
Fournier, MJ
中科院分区：
文献类型：
--
作者：
Balakin, AG;Smith, L;Fournier, MJ
我们发现，除MRP/7-2RNA外，所有已知的酵母和脊椎动物小核仁RNA(SnoRNAs)主要分为两大类。一类由保守的框C和D定义，另一类由一个新的元件定义：共识ACA三联体位于RNA的3‘端之前3个核苷酸。通过对酵母ACA snoRNA(NR11)的突变分析，确定了ACA盒在snoRNA稳定性中的作用。箱体的全部功能取决于相邻上游阀杆的完整性。酵母ACA家族的所有成员都与GAR1蛋白相关。这种或另一种常见的小核仁核糖核蛋白颗粒蛋白的结合被预测为snoRNA转录后生命的关键入口点，包括snoRNA3‘端的精确形成。
We have discovered that all known yeast and vertebrate small nucleolar RNAs (snoRNAs), except for the MRP/7-2 RNA, fall into two major classes. One class is defined by conserved boxes C and D and the other by a novel element: a consensus ACA triplet positioned 3 nt before the 3' end of the RNA. A role for the ACA box in snoRNA stability has been established by mutational analysis of a yeast ACA snoRNA (snR11). Full function of the box depends on the integrity of an adjacent upstream stem. All members of the yeast ACA family are associated with the GAR1 protein. Binding of this or another common small nucleolar ribonucleoprotein particle protein is predicted to be a critical entry point to snoRNA posttranscriptional life, including precise formation of the snoRNA 3' end.