Antenna-based optical imaging of single Ca2+ transmembrane proteins in liquids
Antenna-based optical imaging of single Ca2+ transmembrane proteins in liquids
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DOI：
10.1021/nl073057t
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发表时间：
2008-02-01
期刊：
影响因子：
10.8
通讯作者：
Novotny, Lukas
中科院分区：
文献类型：
--
作者：
Hoppener, Christiane;Novotny, Lukas
了解生物过程的多样性需要能够在其自然环境中处理单个蛋白质的方法，并提供对每个蛋白质的结构和功能特性以及局部分布的见解。我们使用单个金纳米颗粒形式的光学天线将入射激光辐射定位到50 nm，显著小于光的衍射极限。我们的方法使我们能够光学解决个别质膜结合钙泵。（PMCA 4）浸入水性环境中，并确定蛋白质间距离的分布。我们能够将蛋白质图谱与局部拓扑结构相关联。改进的天线几何形状将使其能够以5-10 nm的真实蛋白质分辨率解析、识别和探测活细胞中的单膜蛋白质。
Understanding the diversity of biological processes requires methods that can address single proteins in their natural environment and provide insights into structural and functional properties, as well as the local distribution of each individual protein. We use an optical antenna in the form of a single gold nanoparticle to localize incident laser radiation to 50 nm, significantly smaller than the diffraction limit of light. Our approach enables us to optically resolve individual plasma-membrane-bound Ca2+ pumps. (PMCA4) immersed in aqueous environments and to determine the distribution of interprotein distances. We are able to correlate the protein maps with local topology. Improved antenna geometries will make it possible to resolve, identify, and probe single membrane proteins in live cells with true protein resolution of 5-10 nm.